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2 protocols using rabbit anti p15

1

Immunoblotting of Cell Cycle Regulators

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Immunoblotting was performed using the following antibodies: rabbit anti-Bmi-1 (#5856; Cell Signaling Technology, Inc., Beverly, MA, USA), rabbit anti-p16 (sc1207; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rat anti-p19Arf (sc32748; Santa Cruz Biotechnology, Inc.), rabbit anti-p15 (sc613; Santa Cruz Biotechnology, Inc.), rabbit anti-p18 (sc865; Santa Cruz Biotechnology, Inc.), rabbit anti-phospho-Rb (ser807/811) (#9308; Cell Signaling Technology, Inc.), rabbit anti-phospho-Akt (ser473) (#4060; Cell Signaling Technology, Inc.), rabbit anti-Akt (#4691; Cell Signaling Technology, Inc.), and mouse anti-β-actin (A5316; Sigma-Aldrich, St. Louis, MO, USA).
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2

Comprehensive Antibody Resource for Cell Signaling

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The following antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA): rabbit anti-p53, rabbit anti-Bcl2, rabbit anti-Cdk4, rabbit anti-Bcl2, and rabbit anti-p15. Goat anti-p21 was purchased from R&D Systems (Minneapolis, MN), goat anti-Oct4a, and rabbit anti-Msi-1 from Abcam (Cambridge, MA); mouse anti-Hes1 from NovusBio (Centennial, Colorado); rabbit anti-p16, rabbit anti-phospho-PTEN, mouse anti-Rb, rabbit anti-phospho-PDK1, rabbit anti-PTEN, rabbit anti-PD-L1 and mouse anti-Cyclin E from Cell Signaling Technology (Massachusetts, USA). Cell Signaling kindly provided rabbit anti-phospho AKT as part of a sample kit. Mouse anti-PI3K was purchased from Millipore (St. Louis, MO), mouse anti-β-actin from Sigma-Aldrich, and mouse anti rabbit IgG-Alexafluor 647 from Invitrogen (Thermo Fisher Scientific, Waltham, MA).
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