Luciferin substrate

Manufactured by PerkinElmer

Sourced in United States

Luciferin substrate is a chemical compound used in bioluminescence assays. It serves as a substrate for luciferase enzymes, enabling the production of light through a chemiluminescent reaction. The core function of the Luciferin substrate is to facilitate the detection and measurement of luciferase activity in various biological samples and applications.

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Lab products found in correlation

Xenogen ivis 200, by PerkinElmer (2 mentions) Indigo, by Berthold Technologies (1 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions) Ivis 100, by PerkinElmer (1 mentions) Living image 4, by PerkinElmer (1 mentions)

Close spelling variants of this product

XenoLight d-luciferin substrate XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate Xenolight D-Luciferin-K+ Salt Bioluminscent substrate IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate D-luciferin bioluminescent substrate RediJect D-Luciferin Bioluminescent Substrate Luciferin substrate solution RediJect D-Luciferin Ultra Bioluminescent Substrate XenoLight D-Luciferin Potassium Salt Bioluminescent Substrate XenoLight d-Luciferin-K+ Salt Substrate

7 protocols using luciferin substrate

Preclinical Evaluation of CAR-T Therapies

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Five week old NCG (NOD/ShiLtJGpt-Prkdc^em26Cd52Il2rg^em26Cd22/Gpt) mice were obtained from GemPharmatech (Nanjing, China), and at 6 weeks, they were injected intraperitoneally (i.p.) with 1 × 10⁵ LEL6 cells. 7 days later, the mice were injected with 1 million UTD cells/3B CAR-T cells/3BD CAR-T cells. The second-round and third-round injections of 2 million UTD cells/3B CAR-T cells/3BD CAR-T cells were performed at Day 14 and Day 21 post LEL6 cell injection.
Mice were subjected to weekly bioluminescence imaging. Briefly, mice were anesthetized and injected intraperitoneally (i.p.) with luciferin substrate (150 mg/kg) (Perkin Elmer, Waltham, MA, United States). Luciferase activity was measured within 10 min using NightOW LB983 (Berthold). Data were analyzed and exported using IndiGO (Berthold, Stuttgart, Germany). Luminescence signal intensity is represented by radiance in photons per second per centimeter squared per steradian (p/sec/cm²/sr). Mice were monitored weekly for weight loss and mortality for 70 days. Normally, we assumed that mice die by default when their weight drops by 25%.

Jiang Z., Liang H., Pan H., Liang Y., Wang H., Yang X., Lu P., Zhang X., Yang J., Zhang D., Shen X., Wang J., Liang Z., Lin Q., Wang Y., Zhao L., Zhong Y., Lu H, & Zhu H. (2021). HIV-1-Specific CAR-T Cells With Cell-Intrinsic PD-1 Checkpoint Blockade Enhance Anti-HIV Efficacy in vivo. Frontiers in Microbiology, 12, 684016.

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In Vivo Bioluminescence Imaging

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Mice were imaged on the Xenogen IVIS 200 (Perkin Elmer, Waltham, MA) approximately five minutes after injection with 100 μg of luciferin substrate (Perkin Elmer, Waltham, MA) in phosphate buffered saline without calcium or magnesium (PBS; Corning, Manassas, VA) as previously described19 (link).

Woodard L.E., Cheng J., Welch R.C., Williams F.M., Luo W., Gewin L.S, & Wilson M.H. (2017). Kidney-specific transposon-mediated gene transfer in vivo. Scientific Reports, 7, 44904.

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Longitudinal Live Imaging of Orthotopic ESCC Tumors

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Live imaging was done weekly by using the Xenogen in vivo imaging system, IVIS-100 (Perkin Elmer, MA, USA) to monitor the orthotopic tumor growth kinetics of the luciferase-labelled ESCC cell lines injected into the mice and to observe for metastasis. The 3D live images were captured by using the Xenogen IVIS Spectrum. Luciferin substrate (Perkin Elmer) at 150 mg/kg was injected into the animals prior to bioluminescence imaging. Animals were euthanized at the end of the study at weeks 3 to 5 to excise the orthotopic tumors. The tumors were dissected and fixed in formalin and embedded in paraffin. Sectioned tissues were stained with hematoxylin and eosin for histological examination by pathologists (Kwok Wah Chan and Alfred K. Lam).

Ip J.C., Ko J.M., Yu V.Z., Chan K.W., Lam A.K., Law S., Tong D.K, & Lung M.L. (2015). A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma. BioMed Research International, 2015, 910715.

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Bioluminescence Monitoring of Respiratory Infections

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The rSeV infection of challenged or co-housed mice was quantified by bioluminescence measurement. Every 24 hours mice were injected i.p. with 3 mg luciferin substrate (PerkinElmer) in PBS. After 15 minutes, mice were anesthetized using inhaled isoflurane through a 5-port manifold, placed in supine positions, scanned for bioluminescence by the IVIS CCD camera (Caliper LifeSciences) using auto-exposure and a 23 cm imaging field of view (FOV). Images were analyzed with Living Image 4.3.1 software (PerkinElmer). To quantify bioluminescence, square regions of interest (ROI) were defined manually including the upper and lower respiratory tract (nasopharynx, trachea and lungs) of each animal, quantified and expressed as “Avg Radiance” - numbers of photons emitted per unit time from a defined area (photons per second per square centimeter per steradian, p/s/cm²/sr). Bioluminescence curves were graphed over time, and the area under the curve (AUC) was calculated using GraphPad Prism with one as the baseline.

Zhou J., Uddback I., Kohlmeier J.E., Christensen J.P, & Thomsen A.R. (2023). Vaccine induced memory CD8+ T cells efficiently prevent viral transmission from the respiratory tract. Frontiers in Immunology, 14, 1322536.

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Bioluminescent Imaging of Mice

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Mice were anesthetized using isoflurane and injected i.p. with 100 μg luciferin substrate (Perkin Elmer, Waltham, MA) in PBS. Approximately 10 min after luciferase injections mice were imaged on the Xenogen IVIS 200 (Perkin Elmer, Waltham, MA) as described previously^{22 (link)}. All data shown represent mean luminescence observed by summing dorsal and ventral measurements obtained from identical region of interests drawn over the trunk and head of each individual mouse.

O’Neil R.T., Saha S., Veach R.A., Welch R.C., Woodard L.E., Rooney C.M, & Wilson M.H. (2018). Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo. Nature Communications, 9, 1325.

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Luciferase Complementation Imaging Assay in Nicotiana benthamiana

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LCI assays were conducted as described with minor modifications. The constructs were transformed into A. tumefaciens strain GV3101 using electroporation. The vectors were infiltrated and expressed in the combinations described in N. benthamiana leaves with a final concentration of OD₆₀₀ 1.0 for approximately 48 h. Subsequently, 0.2 mM luciferin substrate (Perkin Elmer, EU) was diluted with 0.01% Triton X-100 and infiltrated into leaves under darkness for 5 min and the luciferase activity was tested using a low-light cooled CCD imaging apparatus (NightOWL II LB983). In the competition LCI assays, the GV3101 strains harboring NLUC-OsNPR1 and CLUC-OsNPR1 with P2-FLAG were co-infiltrated into N. benthamiana leaves, GUS-MYC was co-infiltrated as a negative control. At least three biological repeats were conducted for all experiments.

Zhang H., Wang F., Song W., Yang Z., Li L., Ma Q., Tan X., Wei Z., Li Y., Li J., Yan F., Chen J, & Sun Z. (2023). Different viral effectors suppress hormone-mediated antiviral immunity of rice coordinated by OsNPR1. Nature Communications, 14, 3011.

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Bioluminescence Imaging of Mice

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Mice were anesthetized using isoflurane and injected i.p. with luciferin substrate (PerkinElmer, Waltham, MA, USA) at a standard concentration of 150 mg/kg in PBS. Approximately 10 min after luciferase injections, mice were imaged on the AMI-HT (Spectral Imaging, Tucson, AZ, USA). All data shown represent mean luminescence observed by summing dorsal and ventral measurements obtained from identical regions of interest drawn over the trunk and head of each individual mouse.

Tennant M.D., New C., Ferreira L.M.R, & O'Neil R.T. (2023). Efficient T cell adoptive transfer in lymphoreplete hosts mediated by transient activation of Stat5 signaling. Molecular therapy : the journal of the American Society of Gene Therapy, 31(9).

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