C57bl 6j b6 h 2b

Male C57BL/6J (B6; H-2^b) and BALB/c (H-2^d) mice (aged 8–10 wk) were purchased from the Charles River (Beijing, China). Lyz2Cre mice (C57BL/6 background) and B6.C-H-2bm12 (Bm12) mice were purchased from Jackson Laboratory (USA). Map2k1 and Map2k2 flox mice were generated using Shanghai Model Organisms (Shanghai, China) on a C57BL/6 background. Specifically, the Map2k1 flox mice were obtained by transcription activator-like effector nucleases–mediated insertion of loxP sites in the Map2k1 gene, whereas the Map2k2 flox mice were obtained by CRISPR/Cas9-mediated insertion of loxP sites in the Map2k2 gene. Macrophage-specific Map2k1 and Map2k2 double-gene knockout (DKO) mice were generated by crossing Map2k1 and Map2k2 flox mice with Lyz2Cre mice. The genotype primer sequences of these mice are listed in Table S3 (SDC, http://links.lww.com/TP/C952). Animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of Huazhong University of Science and Technology. All mouse experiments were performed in a special pathogen-free facility according to the Animal Care and Use Committee of Huazhong University of Science and Technology guidelines and in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. CO₂ asphyxiation was performed for euthanasia.