Anti snail antibody

Triton X-100, Tris-HCl, puromycin, Trypan blue EDTA, ribonuclease-A, and dimethyl sulfoxide (DMSO) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). The anti-snail antibody was purchased from GenScript. The anti-p-snail and anti-Hsp27 antibodies were purchased from Abcam. Antibodies against IGFBP2, β-catenin, vimentin, and GAPDH were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Hep-J5 and Mahlavu cells were gifts from Dr. C. S. Yang, National Taiwan University, and Dr. C. P. Hu, Veterans General Hospital, Taiwan [20 (link), 36 (link), 37 (link)]. The HCC cell lines were grown in Dulbecco's modified Eagle's medium (DMEM, Gibco BRL, Grand Island, NY, USA) supplemented with 2 mM L-glutamine, 1.5 g/l sodium bicarbonate, 10% fetal calf serum (FCS; Gibco BRL) and 2% penicillin-streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin). The cells were maintained in a 5% CO₂ humidified incubator at 37°C as previously described [38 (link), 39 (link)].

Propidium iodide (PI), Tris-HCl, trypan blue, ethylenediaminetetraacetic acid (EDTA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ribonuclease A, and dimethyl sulfoxide (DMSO) were obtained from Sigma Chemical (St. Louis, MO, USA). Anti-HDA6 antibody was purchased from Novus Biologicals, LLC (Centennial, CO, USA). Anti-serpine-1, EGFR, β-catenin, vimentin, Cyclin-dependent kinase 2 (CDK2), fibronectin, Poly (ADP-ribose) polymerase (PARP), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-p38, phospho-p38, c-Jun N-terminal kinases (JNK), phospho-JNK, extracellular regulated protein kinases (ERK), and phospho-ERK antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-snail antibody was purchased form GenScript (Piscataway, NJ, USA). Anti-phospho-snail and Cox-2 antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-cyclin A2 and cyclin B1 were purchased from GeneTex, Inc. (Irvine, CA, USA).