Fitc conjugated mouse anti human cd44

Manufactured by BD

Sourced in United States

FITC-conjugated mouse anti-human CD44 is a labelled antibody that recognizes the CD44 cell surface glycoprotein, which is involved in cell-cell and cell-matrix interactions. The FITC (fluorescein isothiocyanate) label allows for the detection and analysis of CD44-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Reserpine, by Merck Group (1 mentions) Pe conjugated mouse anti human cd24, by BD (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Cellquest pro, by BD (1 mentions) Facsvantage, by BD (1 mentions) Aldefluor assay, by STEMCELL (1 mentions) Fascalibur, by BD (1 mentions) Pe conjugated mouse anti human cd34, by BD (1 mentions) Pe conjugated mouse anti human cd73, by BD (1 mentions) Fitc conjugated mouse anti human cd45, by BD (1 mentions) Phycoerythrin conjugated pe mouse anti human cd29, by BD (1 mentions) Pe conjugated mouse anti human cd90, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Cellquest software, by BD (1 mentions) Apc conjugated anti human cd133, by Miltenyi Biotec (1 mentions) Aldefluor kit, by STEMCELL (1 mentions) Anti v5 antibody, by Abcam (1 mentions) Goat anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm, by BD (1 mentions)

Close spelling variants of this product

FITC-conjugated mouse anti-human CD44 antibodies FITC-conjugated anti-human CD44 FITC-conjugated anti-mouse CD44 FITC mouse anti-human CD44 FITC-conjugated anti-CD44 Anti-human CD44-FITC Mouse anti-human CD44 FITC-conjugated CD44 Anti-CD44-FITC Mouse anti-CD44

6 protocols using fitc conjugated mouse anti human cd44

Characterizing Cancer Stem Cells via Flow Cytometry

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Cells (10⁶ in 1% bovine serum albumin in PBS) were stained with FITC-conjugated mouse anti-human CD44 and R-Phycoerythrin-conjugated mouse anti-human CD24 (BD Bioscience, Oxford, UK) at 1/100 dilution at 4 °C for 30 min. Aldehyde dehydrogenase activity was measured using the ALDEFLUOR assay (STEMCELL Technologies, Grenoble, France). Cells were incubated in ALDEFLUOR reagent with or without DEAB (ALDH inhibitor) at 37 °C for 40 min, centrifuged and re-suspended in assay buffer. In some experiments, PE-conjugated mouse anti-human CD24 (BD Bioscience, Oxford, UK) and Alexa Fluor 647-CD44 (AbD Serotec, Kidlington, UK) were added. For assessment of side-population, cells were stained with Hoechst 33342 (5 μg ml⁻¹) at 37 ^°C for 90 min with or without reserpine (10 μM, as a negative control; Sigma-Aldrich, Dorset, UK), washed and re-suspended in PBS. CellQuest Pro (BD) and Summit v.4 (Dako, Glostrup, Denmark) software were applied for data acquisition and analysis, respectively, using measurements from 10 000 cells in each experiment. Cells were sorted using a FACSVantage (BD Bioscience) either directly into tissue culture plates or into centrifuge tubes for further culture.

Liu Y., Nenutil R., Appleyard M.V., Murray K., Boylan M., Thompson A.M, & Coates P.J. (2014). Lack of correlation of stem cell markers in breast cancer stem cells. British Journal of Cancer, 110(8), 2063-2071.

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Astrocyte CD44 Expression Analysis

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HA1800 astrocytes were dissociated into single cells using TrypLE and then washed with cold PBS containing 0.5% BSA and 2 mM EDTA. Subsequently, the astrocytes were incubated with FITC-conjugated mouse anti-human CD44 or FITC-conjugated mouse IgG2a, κ isotype control (BD Bioscience, USA) for 30 min at 4 °C in the dark before being washed 3 times with cold PBS buffer. FASCalibur (BD Biosciences) and Flowjo software were used to acquire and analyze data.

Zhao A.D., Qin H., Sun M.L., Ma K, & Fu X.B. (2020). Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules. Military Medical Research, 7, 42.

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Characterization of ATMSC Surface Markers

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Flow cytometry was used to analyze the expression of cell surface markers in RFP- and Oct4/Sox2-ATMSCs. By passage 5, a homogenous population of rapidly dividing cells with fibroblastoid morphology was obtained. ATMSCs were fixed with 70% ethanol at 4°C and stained for 30 min on ice with primary antibodies that recognized various surface molecules. Phycoerythrin-conjugated (PE) mouse anti-human CD29 (BD Bioscience, San Jose, CA), fluorescein isothiocyanate-conjugated (FITC) mouse anti-human CD31 (BD Bioscience), PE-conjugated mouse antihuman CD34 (BD Bioscience), FITC-conjugated mouse anti-human CD44 (BD Bioscience), FITC-conjugated mouse anti-human CD45 (BD Bioscience), PE-conjugated mouse anti-human CD73 (BD Biosciences Pharmingen, San Diego, CA), PE-conjugated mouse anti human CD90 (BD Biosciences), and PE-conjugated antihuman CD105/endoglin (R&D System, Minneapolis, MN) were used for the detection of cell surface antigens. The immunophenotype of MSCs was analyzed with the FACSCalibur flow cytometer (BD Biosciences, Bedford, MA) using the CELLQuest software (BD Biosciences).

Han S.M., Coh Y.R., Ahn J.O., Jang G., Yum S.Y., Kang S.K., Lee H.W, & Youn H.Y. (2015). Enhanced Hepatogenic Transdifferentiation of Human Adipose Tissue Mesenchymal Stem Cells by Gene Engineering with Oct4 and Sox2. PLoS ONE, 10(3), e0108874.

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Surface Marker Expression in Cancer Cells

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The surface expression of CD133, CD44, and CD24 was analyzed by immunophenotyping. Briefly, 1X10⁶ cells were seeded in a 10 cm dish and incubated for 24 h in CO₂ incubator at 37°C prior to treatment. Cells were treated with TXL, APC, and TG alone or in combinations for 24 h. Cells were labeled with APC-conjugated anti-human CD133 (Miltenyi Biotec, San Diego, USA). FITC-conjugated mouse anti-human CD44 (BD Pharmingen, BD Biosciences, USA) and/or APC-conjugated CD24 in a buffer containing (1X PBS, 0.5% BSA and 2 mM EDTA) for 15 min at 4°C. Labeled cells were re-suspended in 1X PBS (pH 7.4), and analyzed by flow cytometer (LSR II A, BD Bioscience). Unstained cells served as negative controls. A total of 100,000 events were capture for each sample. Estimation of ALDH bright (ALDH^br) cell populations was performed 24 h after treatment using the Aldefluor^™ kit (Stem Cell Technology). Data were plotted using Win List 3D software.

Kumar S., Chaudhary A.K., Kumar R., O'Malley J., Dubrovska A., Wang X., Yadav N., Goodrich D.W, & Chandra D. (2016). Combination therapy induces unfolded protein response and cytoskeletal rearrangement leading to mitochondrial apoptosis in prostate cancer. Molecular Oncology, 10(7), 949-965.

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Validating MLL1-ZC3H13 Fusion Expression

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The isolated clones (V5‐tagged) were tested for the expression of MLL1‐ZC3H13 fusion construct by flow cytometry using the anti‐V5 antibody. Along with clones, a parental control and vector control were also used for the validation assays. Briefly, for flow cytometry, the single‐cell population of clones and controls were fixed and permeabilized using reagents from the kit following the manufacturer's suggestion (BD Cytofix/Cytoperm, BD Biosciences, Mississauga, Canada; 554714). The cells were stained with anti‐V5 antibody (Abcam, Toronto, Canada, AB9116) and then with secondary goat anti‐rabbit IgG antibody conjugated with AF488 (Thermo Fisher Scientific, A11008). The data were subsequently analyzed by flowjo^® software (FlowJo LLC., Ashland, OR, USA, version 9.9 for Mac). The stemness of the clones was also assessed by flow cytometry following direct staining of cells without fixation or permeabilization using FITC‐conjugated mouse anti‐Human CD44 (BD Biosciences, 555478) along with Isotype control (FITC‐conjugated Mouse IgG2b Κ‐BD Biosciences, 555742).

Parameswaran S., Vizeacoumar F.S., Kalyanasundaram Bhanumathy K., Qin F., Islam M.F., Toosi B.M., Cunningham C.E., Mousseau D.D., Uppalapati M.C., Stirling P.C., Wu Y., Bonham K., Freywald A., Li H, & Vizeacoumar F.J. (2018). Molecular characterization of an MLL1 fusion and its role in chromosomal instability. Molecular Oncology, 13(2), 422-440.

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Characterization of Stem Cell Surface Markers

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cPDLs (1.5 × 10⁷ cells) were washed with 1% PBS and then incubated with antibodies. The antibodies were FITC conjugated mouse anti-human CD44 (BD Bioscience Pharmingen, USA), PerCP conjugated mouse anti-human CD45 (Immuno Tools, Germany), APC conjugated mouse anti-human CD90 (Immuno Tools, Germany), and PE conjugated mouse anti-human CD105 (Immuno Tools, Germany). Cells were analyzed using FACsCalibur using the CellQuest software (BD CellQuest Pro 6.1, BD Bioscience, USA).

Banyatworakul P., Osathanon T., Chumprasert S., Pavasant P, & Pirarat N. (2021). Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro. Scientific Reports, 11, 11409.

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