SH-SY5Y cell line (Korean Cell Line Bank, 22266) were maintained in DMEM/F12 (Gibco, 11320033) supplemented with 10% fetal bovine serum (Welgene, S001-01) containing Penicillin/Streptomycin (Lonza, 17-602E) at 5% CO2 in 37 °C. HCT116 cell line and DNMT1 (Δexons3-5/Δexons3-5); DNMT3B (−/−) of HCT116 (horizon, HD R02-079), named as DKO1, were maintained in RPMI 1640 (Sigma, R8758), supplemented with 10% FBS (Welgene, S001-01) containing Penicillin/Streptomycin (Lonza, 17-602E) at 5% CO2 in 37 °C. 293T cell line were maintained in DMEM (Welgene, LM 001-05) supplemented with 10% FBS(Welgene, S001-01) containing Penicillin/Streptomycin (Lonza, 17-602E) at 5% CO2 in 37 °C. Drosophila S2 cells (ATCC, CRL-1963) were cultured in Schneider’s Drosophila Medium (Gibco, 21720024) supplemented with 10% FBS (Welgene, S001-01) in 25 °C.
Sh sy5y cell line
Manufactured by Korean Cell Line Bank
The SH-SY5Y cell line is a commonly used human-derived neuroblastoma cell line. It is a subclone of the SK-N-SH cell line, originally isolated from a bone marrow biopsy of a 4-year-old female with neuroblastoma. The SH-SY5Y cell line retains many of the characteristics of mature neurons and is widely used in research on neurodegenerative diseases, neurotoxicology, and neuronal differentiation.
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Lab products found in correlation
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Schneider s drosophila medium, by Thermo Fisher Scientific (1 mentions)
Drosophila s2 cells, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Pc12 cell line, by American Type Culture Collection (1 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Lonza (1 mentions)
Sodium pyruvate, by Merck Group (1 mentions)
7 protocols using sh sy5y cell line
1
Culturing Cell Lines for Research
2
Neuroprotective Effects of hkRA in SH-SY5Y Cells
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The human neuroblastoma SH-SY5Y cell line was purchased from the Korean Cell Line Bank (Republic of Korea ). SH-SY5Y cells were cultured in DMEM supplemented with high glucose containing 10% fetal bovine serum (FBS) and penicillin–streptomycin (100 U/ml each) (HyClone, USA) at 37°C in 5% CO2. All experiments were performed 24 h after seeding the cells onto the plates. SH-SY5Y cells were pretreated with various concentrations (106, 107, and 108 cells/ml) of hkRA for 30 min and then exposed to 10 μM Aβ25–35 dissolved in distilled water for 24 h. Only cell passages under 30 were used in all experiments.
3
Culturing Rat and Human Neuronal Cell Lines
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The PC-12 cell line, derived from a rat pheochromocytoma, was obtained from the American Type Culture Collection (USA). The SH-SY5Y cell line, derived from a human neuroblastoma, was obtained from the Korean Cell Line Bank (Republic of Korea). The PC-12 cell line was cultured in RPMI 1640 medium containing 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin, and the SH-SY5Y cells were cultured in MEM medium containing 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. Two neuronal cell lines (PC-12 and SH-SY5Y) were incubated in a humidified incubator (CO2 incubator BB 15; Thermo Electron LED GmbH, Germany) with 5% CO2 at 37°C.
4
Neuroblastoma SH-SY5Y Cell Culture and Treatment
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The human neuroblastoma SH-SY5Y cell line was purchased from the Korean Cell Line Bank (KCLB) of Seoul National University (Seoul, Korea) and cultured in DMEM containing 10% FBS, as well as an antibiotic–antimycotic solution containing 100 μg/mL streptomycin, 100 U/mL penicillin, and 0.25 μg/mL Fungizone. The cells were incubated in a humidified atmosphere with 5% CO2 at 37 °C. The cells were harvested using 0.25% trypsin EDTA and were sub-cultured into 100 mm culture dishes. Grown cells were seeded into 100 mm dishes and 24- and 96-well plates. TMT was dissolved in dimethyl sulfoxide (DMSO). Each control was added to the same volume of DMSO as a vehicle-treated group. Cells were exposed to TMT for 12 h for TEM observation and for 24 h for other analyses. Esculetin, meloxicam, celecoxib, and phenidone were pre-incubated for 2 h prior to TMT treatment and maintained in co-exposure with TMT and test chemicals for 24 h. Other test chemicals were co-exposed with TMT for 24 h. Toxicity was evaluated at the highest concentration of each test chemical.
5
Neuroblastoma Cell Culture and Amyloid Beta Treatment
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Human neuroblastoma SH-SY5Y cell line was obtained from Korean cell line bank (Seoul, Korea). The cells were cultured in DMEM supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 U/mL). Cells were incubated in a humidified 5% CO2 incubator at 37°C and grown in 6-well plates for 3 days (72 h) with or without CORT (3 nM and 30 nM). Then, the cells were plated at an appropriate density based on each experimental scale. For the treatment of Aβ25-35, the cells were switched to the serum-free medium and incubated with or without Aβ25-35 for indicated times.
6
Culture of SH-SY5Y Dopaminergic Neuroblastoma
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The human dopaminergic neuroblastoma SH-SY5Y cell line was obtained from KCLB (Korean Cell Line Bank, Seoul, Korea) and grown in DMEM/F-12 (Gibco, Grand Island, NY) containing 10% heat-inactivated fetal bovine serum (Gibco) and 1% (100 U/ml) penicillin-streptomycin in humidified incubator with 5% CO2 at 37°C.
7
SH-SY5Y Cell Culture Protocol
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The human neuroblastoma SH-SY5Y cell line was obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in Eagle’s minimum essential medium (EMEM, Lonza, Basel, Switzerland) supplemented with 50% F-12 (Lonza, Basel, Switzerland), 10% fetal bovine serum (Alphabioregen, Boston, MA, USA), 1% penicillin and streptomycin (Gibco, Waltham, MA, USA), 1× non-essential amino acid (NEAA, Lonza, Basel, Switzerland), and 1 mM sodium pyruvate (Sigma-Aldrich, St. Louis, MO, USA).
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