Coagulation tests, including PT, aPTT, and factor assays, were performed on an automated coagulation analyzer, ACL TOP (Instrumentation Laboratory, Lexington, MA, USA). PT was measured using the HemosIL RecombiPlasTin reagent (Instrumentation Laboratory,), and aPTT was measured using the SynthASil reagent (Instrumentation Laboratory). Fibrinogen was measured using the HemosIL Fibrinogen-C XL reagent (Instrumentation Laboratory) based on the Clauss method. Coagulation factors were tested using a PT-based clotting assay with the HemosIL RecombiPlasTin reagent (for FV) and an aPTT-based clotting assay using the SynthASil reagent (for FVIII, FXI, and FXII). Antithrombin and protein C activity were determined with chromogenic assays (HemosIL liquid antithrombin and HemosIL Protein C; Instrumentation Laboratory) and free protein S antigen with an immunoassay (HemosIL Free Protein S, Instrumentation Laboratory).
Hemosil fibrinogen c xl reagent
Manufactured by Werfen
The HemosIL Fibrinogen-C XL reagent is a laboratory diagnostic tool used for the quantitative determination of fibrinogen levels in human plasma. It provides a standardized and reproducible method for assessing fibrinogen concentration, which is an important coagulation factor.
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Lab products found in correlation
Hemosil liquid antithrombin, by Werfen (2 mentions)
Hemosil protein c, by Werfen (2 mentions)
Synthasil reagent, by Werfen (1 mentions)
Hemosil free protein s, by Werfen (1 mentions)
Acl top, by Werfen (1 mentions)
Recombiplastin, by Werfen (1 mentions)
Synthasil, by Werfen (1 mentions)
Acl 3000, by Werfen (1 mentions)
2 protocols using hemosil fibrinogen c xl reagent
1
Comprehensive Coagulation Profile Analysis
2
Coagulation Assays: A Comprehensive Approach
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Both PT and aPTT were assayed using the RecombiPlasTin (Instrumentation Laboratory, Milan, Italy) and SynthASil (Instrumentation Laboratory) on an ACL 3000 (Instrumentation Laboratory). Fibrinogen was measured using the HemosIL Fibrinogen-C XL reagent (Instrumentation Laboratory SpA). Coagulation factors were assayed using a PT-based clotting assay with the HemosIL RecombiPlasTin reagent (for FII, FV, FVII, and FX) and an aPTT-based clotting assay using the SynthASil reagent (for FVIII, FXI, FXI, and FXII). d -dimers were measured using an immunoturbidimetric method on ACL 3000. Antithrombin and protein C activity was determined using chromogenic assays (HemosIL liquid antithrombin and HemosIL protein C, respectively; Instrumentation Laboratory SpA). The tissue factor pathway inhibitor (TFPI) was measured using a TFPI Human ELISA Kit (Abcam (Cambridge, MA)) according to the manufacturer’s protocol.
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