Blood was collected from the tip of the tails, and blood glucose levels were measured with an Antsense Duo Small Electrode Glucose Analyzer (Horiba, Kyoto, Japan). Blood samples were centrifuged (13,500 rpm, 10 min, 4 °C) twice, and the collected plasma samples were stored at −80 °C until the biochemical analyses. Plasma hormone levels were measured using the following assays: insulin, Mouse/Rat Insulin ELISA Kit (Morinaga Institute of Biological Science, Kanagawa, Japan); FGF21, Mouse and Rat FGF21 ELISA Kit (BioVendor Inc., Brno, Czech Republic); GCG, Glucagon ELISA Kit (Mercodia, Uppsala, Sweden); active GLP-1, GLP-1 Active Form Assay Kit (IBL, Gunma, Japan); GIP, GIP (total) ELISA kit (Millipore, Billerica, MA, USA); and corticosterone, Corticosterone Enzyme Immunoassay Kit (Arbor Assays, Ann Arbor, MI, USA). Plasma amino acid concentrations were measured by standard assays at SRL, Tokyo, Japan [21 (link)].
Antsense duo small electrode glucose analyzer
Manufactured by Horiba
Sourced in Japan
The Antsense Duo Small Electrode Glucose Analyzer is a compact device designed for the measurement of glucose levels. It utilizes small electrode technology to provide accurate and reliable glucose readings.
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Lab products found in correlation
2 protocols using antsense duo small electrode glucose analyzer
1
Plasma Hormone and Amino Acid Analysis
2
Plasma Glucose and Insulin Analysis
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Blood was collected from the tip of the tail, and blood glucose levels were measured by an Antsense Duo Small Electrode Glucose Analyzer (Horiba, Kyoto, Japan). Blood samples were centrifuged (2,000 g, 15 min, 4°C) and the collected plasma samples were stored at −80°C until analysis. Plasma insulin levels were measured using a Mouse/Rat Insulin ELISA Kit (Morinaga Institute of Biological Science, Kanagawa, Japan), and plasma amino acid concentrations were measured by standard assays at SRL, Tokyo, Japan28 , 31 .
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