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Rabbit anti iκβα antibody

Manufactured by Merck Group
Sourced in United States

The Rabbit anti-IκΒα antibody is a research-use laboratory reagent that recognizes the IκΒα protein. IκΒα is an inhibitor of the NF-κB transcription factor, which plays a crucial role in cellular processes such as inflammation, immune response, and cell survival. This antibody can be used to detect and quantify IκΒα levels in various experimental systems.

Automatically generated - may contain errors

2 protocols using rabbit anti iκβα antibody

1

Investigating Anti-Cancer Mechanisms

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All cell culture media, antibiotics, and trypsin were purchased from Gibco (Grand Island, NY, USA), and foetal bovine serum (FBS) was purchased from HyClone (Logan, UT, USA). Rabbit anti-NF-κΒ p65 antibody, rabbit anti-IKKα antibody, rabbit anti-IκΒα antibody, rabbit anti-caspase-8 antibody, rabbit anti-GAPDH antibody, rabbit anti-Poly[ADP-ribose] Polymerase (PARP) antibody, rabbit anti-cyclin B1 antibody, rabbit anti-survivin antibody, rabbit anti-caspase-3 antibody, mouse anti-α-tubulin antibody, rabbit anti-β-actin antibody, goat anti-rabbit IgG-peroxidase, goat anti-mouse IgG-peroxidase, goat anti-rabbit IgG-FITC, goat anti-mouse IgG-FITC, DAPI, methyl thiazolyl tetrazolium (MTT), and Taxol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Immobilon membranes were purchased from Merck Millipore (Bedford, MA, USA). Cell Tracker CM-Dil and Calcein-AM were purchased from Invitrogen (Carlsbad, CA, USA). ECL Plus substrate, bicinchoninic acid (BCA) reagents, and RIPA lysis buffer were purchased from CWBio (Beijing, China). Kanglaite injection was purchased from Zhejiang Kanglaite Pharmaceutical Co., Ltd, (Zhejiang, China).
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2

Evaluating Anti-cancer Mechanisms of KLT Injection

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All cell culture media, antibiotics, and trypsin were purchased from Gibco (Grand Island, NY, USA), and fetal bovine serum (FBS) was purchased from HyClone (Logan, UT, USA). Mouse anti-E-cadherin antibody, mouse anti-NF-κΒ p65 antibody, mouse anti-cyclin D1 antibody, mouse anti-c-myc antibody, and mouse anti-snail antibody were purchased from Santa Cruz Biotechnology (CA, USA). Rabbit anti-matrix metalloproteinase-9 (MMP-9) antibody, rabbit anti-IKKα antibody, rabbit anti-IκΒα antibody, rabbit anti-vimentin antibody, rabbit anti-Histone H3 antibody, mouse anti-β-tubulin antibody, goat anti-rabbit IgG-peroxidase, goat anti-mouse IgG-peroxidase, goat anti-mouse IgG FITC, goat anti-rabbit IgG FITC, goat anti-mouse IgG TRITC, DAPI and methyl thiazolyl tetrazolium (MTT), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Immobilon membranes were purchased from Merck Millipore (Bedford, MA, USA). ECL Plus substrate, bicinchoninic acid reagents, and RIPA lysis buffer were purchased from CWBio (Beijing, China). TNF-α was purchased from Sino biological Inc. (Beijing, China). KLT injection was purchased from Zhejiang Kanglaite Pharmaceutical Co., Ltd, (Zhejiang, China). Nuclear and cytoplasmic protein extraction kit, crystal violet were purchased from Beyotime (Shanghai, China).
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