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Spectrapor dialysis membrane

Manufactured by Serva Electrophoresis
Sourced in Germany

Spectrapor® dialysis membrane is a semi-permeable membrane used for dialysis. It allows the passage of small molecules while retaining larger molecules and particles.

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2 protocols using spectrapor dialysis membrane

1

Lipid-based Nanoparticle Formulation

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1,2-distearoyl-sn-glycerol-3-phosphatidylcholine (PC), 1,2-distearoyl-sn-glycero-3-phospho-(19-rac-glycerol) (sodium salt) (PG), and 1,2-Distearoyl-sn-glycerol-3-phosphatidyl-ethanolamine-N-[methoxy (polyethylene-glycol)-2000] (PEG) were purchased from Lipoid, Ludwigshafen am Rhein, Germany. Cholesterol (Chol) was purchased from Sigma–Aldrich (Darmstadt, Germany). Dapto was purchased from Tocris Bioscience. All solvents and chromatography materials used were of analytical or HPLC grade and purchased from Merck (Darmstadt, Germany). All other materials, such as salts used for buffer preparation, reagents for lipid concentration determination, etc., were of analytical grade and were purchased from Sigma–Aldrich (Darmstadt, Germany). Spectrapor® dialysis membrane with an MWCO of 12–14 kDa was from Serva, Heidelberg, Germany. Materials used for antimicrobial activity evaluation are mentioned in the sections of the specific methods applied (below).
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2

Liposomal Formulation of Moxifloxacin

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Egg phosphatidylocholine (PC), 1,2-Distearoyl-sn-glycerol-3-phosphatidyl-ethanolamine-N-[methoxy (polyethylene-glycol)-2000] (PEG), and Phosphatidyl-glycerine (PG) were purchased from Lipoid, Germany. Cholesterol (Chol) was purchased from Sigma-Aldrich (Darmstadt, Germany). Moxifloxacin was kindly supplied by COOPER (Athens, Greece). Agar was from Sigma-Aldrich (Darmstadt, Germany). All solvents used were of analytical or HPLC grade and purchased from Merck (Darmstadt, Germany). All other materials, such as salts used for buffer preparation, reagents for lipid concentration determination, etc., were of analytical grade and were purchased from Sigma–Aldrich (Darmstadt, Germany). Spectrapor® dialysis membrane with an MWCO of 12–14 kDa was from Serva, Germany.
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