Rpmi 1640 broth medium

Dimethyl sulfoxide (DMSO), sodium chloride (NaCl), 3-(N-morpholino) propanesulfonic acid (MOPS), N-acetylglucosamine, thiazolyl blue tetrazolium bromide (MTT), ergosterol, amphotericin B and fluconazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). Proline was purchased from Fluka-Sigma-Aldrich (St. Louis, MO, USA). Ethanol and potassium hydroxide (KOH) were purchased from Panreac (Barcelona, Spain). HPLC-grade dichloromethane, n-heptane and ascorbic acid (AA) were purchased from Merck (Darmstadt, Germany). Sabouraud dextrose agar (SDA) and Sabouraud dextrose broth (SDB) were purchased from Bio-Mèrieux (Marcy L’Etoile, France). RPMI-1640 broth medium (with L-glutamine, without bicarbonate and with the pH indicator phenol red) was purchased from Biochrom AG (Berlin, Germany). Yeast nitrogen base was purchased from Difco (New Jersey, USA). Phosphate buffered saline (PBS) was purchased from Fisher Reagent (Geel, Belgium). Hydrogen peroxide (H₂O₂) was purchased from VWR (Fontenay-sous-Bois, France). 2’,7’-Dichlorodihydrofluorescein diacetate (H2DCFDA) was purchased from ThermoFisher Scientific (Life Technologies, Porto, Portugal).

Dimethyl sulfoxide (DMSO), 3-(N-morpholino) propane-sulfonic acid (MOPS), resazurin (REZ), tert-butyl hydroperoxide (t-BHP), carbonyl cyanide m-chlorophenyl hydrazone (CCCP), luciferase from Photinus pyralis (firefly) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640 broth medium (with L-glutamine, without bicarbonate and with the pH indicator phenol red) was purchased from Biochrom AG (Berlin, Germany). Phosphate buffered saline (PBS) was purchased from Fisher Reagent (Geel, Belgium). Potassium hydrogen carbonate (KHCO₃), sodium dihydrogen phosphate dihydrate (Na₂H₂PO₄.2H₂O), adenosine 5′-triphosphate disodium salt hydrate and JC-1 were acquired from Thermo Fisher Scientific (Franklin, MA, USA). Perchloric acid 70% (HClO₄) and Titriplex III (EDTA, disodium salt dihydrate) were obtained from Merck (Darmstadt, Germany). Sodium hydroxide (NaOH) was obtained from VWR (Fontenay-sous-Bois, France). D-Luciferin sodium salt was obtained from Abcam (Cambridge, United Kingdom).
Cyclam salt H₄[H₂(^4-CF3PhCH₂)₂Cyclam]Cl₄ (compound 1) was synthesized according to a previously published procedure [22 (link)].

Dimethyl sulfoxide (DMSO) was obtained from Sigma (Darmstadt, Germany). Mueller–Hinton Broth (MHB) and Mueller–Hinton Agar (MHA) media were purchased from Liofilchem (Teramo, Italy). RPMI-1640 broth medium was obtained from Biochrom AG (Berlin, Germany). Sabouraud dextrose agar (SDA) was purchased from BioMérieux (Marcy L’Étoile, France). 2,2′-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), horseradish peroxidase (HRP) type VI, and 2,2-diphenyl-1-picrylhydrazyl 95% (DPPH) were obtained from Sigma Chem. Co. (Madrid, Spain). H₂O₂ (30% v/v) was purchased from Aldrich Chem. Co. (Madrid, Spain). Methanol, N-O-bis(trimethylsilyl) trifluoroacetamide (BSTFA), and pyridine were obtained from Merck KGaA (Darmstadt, Germany).