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3 protocols using cxcl10 quantikine elisa kit

1

HUVEC Inflammatory Response Characterization

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Human umbilical vascular endothelial cells (HUVECs) were purchased from American Tissue Type Collection. Endothelial Growth Medium (EGM) 2 was obtained from Lonza. AlexaFluor 647-conjugated anti-ICAM1, phycoerythryin-conjugated anti-VCAM1, anti-CXCL10, phycoerythryin/Cy7-conjugated anti-CD14 antibodies and the protein transport inhibitor monensin were purchased from BioLegend. Phycoerythryin-Cy5-conjugated anti-E-selectin and APC-conjugated anti-CD19 antibodies were obtained from BD Biosciences. Qdot 605-conjugated anti-CD3 and Live/Dead fixable violet dead stain kit were from Life Technologies. Recombinant human TNFα and IFNγ, and CXCL10 Quantikine ELISA kit were purchased from R & D Systems.
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2

Quantifying Serum Inflammatory Markers

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Serum levels of CXCL9, CXCL10, and CXCL11 were detected using the mouse CXCL9 Quantikine ELISA kit (Cat # MCX900), CXCL10 Quantikine ELISA kit (Cat # MCX100) and the mouse CXCL11 DuoSet ELISA kit (Cat # DY572), all from R&D Systems (Minneapolis, MN) using the protocol provided by the manufacturer. Serum levels of insulin were detected using the Ultrasensitive Mouse Insulin ELISA kit (Cat # 10-1249-01) from Mercodia (Uppsala, Sweden) according to their recommended procedures.
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3

HUVEC Inflammatory Response Characterization

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Human umbilical vascular endothelial cells (HUVECs) were purchased from American Tissue Type Collection. Endothelial Growth Medium (EGM) 2 was obtained from Lonza. AlexaFluor 647-conjugated anti-ICAM1, phycoerythryin-conjugated anti-VCAM1, anti-CXCL10, phycoerythryin/Cy7-conjugated anti-CD14 antibodies and the protein transport inhibitor monensin were purchased from BioLegend. Phycoerythryin-Cy5-conjugated anti-E-selectin and APC-conjugated anti-CD19 antibodies were obtained from BD Biosciences. Qdot 605-conjugated anti-CD3 and Live/Dead fixable violet dead stain kit were from Life Technologies. Recombinant human TNFα and IFNγ, and CXCL10 Quantikine ELISA kit were purchased from R & D Systems.
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