Elisa kits specific for mice

Serum alanine transaminase (ALT) activities were measured spectrophotometrically using a commercially available kit (Youngdong Pharmaceutical Co., Yongin, Korea) in accordance with the manufacturer's instructions. Serum levels of TNF-α, IL-6, and monocyte chemotactic protein-1 (MCP-1) were determined using enzyme-linked immunosorbent assay (ELISA) kits specific for mice (eBioscience, Vienna, Austria).

Serum glucose, triglyceride, and cholesterol levels were measured by an enzymatic method using commercial kits (Asan Pharmaceutical Co., Seoul, Korea). Serum leptin and adiponectin were measured by enzyme-linked immunosorbent assay (ELISA) using commercial assay kits (BioVendor Laboratorní Medicina a.s, Modrice, the Czech Republic). Serum levels of TNF-α, IL-6, and MCP-1 were also determined using ELISA kits specific for mice (eBioscience, Vienna, Austria). Serum insulin was measured with a commercial ELISA kit (Shibayagi Co., Ltd. Ishihara Shibukawa, Gunma, Japan). Homeostasis model assessment for insulin resistance (HOMA-IR) values were estimated as follows: HOMA-IR = [fasting insulin (µU/mL) × fasting glucose (mmol/L)]/22.5 [27 (link)].