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Dylight 488 coniugated affinipure donkey anti mouse igg

Manufactured by Jackson ImmunoResearch
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DyLight 488-conjugated AffiniPure Donkey Anti-mouse IgG is a fluorescent-labeled secondary antibody produced in donkey and affinity purified. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various applications.

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Lab products found in correlation

Axiovert 200m microscope, by Zeiss (2 mentions) Tra 1 81, by Abcam (2 mentions) Tra 1 60, by Abcam (2 mentions) Nanog, by Abcam (2 mentions) Dylight 549 coniugated affinipure goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions)

Close spelling variants of this product

DyLight 488 (111 citations) Donkey anti-mouse 488 (19 citations) Dylight 488 donkey anti-mouse (3 citations) DyLight 488 anti-mouse (2 citations)

2 protocols using dylight 488 coniugated affinipure donkey anti mouse igg

1

Pluripotency and Lineage Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown in six-well plates and fixed with 4% paraformaldehyde. The following antibodies were used: OCT4 (1:200; Abcam 19857), NANOG (1:200; Abcam 21624), Tra1-81 (1:200; Abcam 16289), Tra1–60 (1:200; Abcam 16288), Smooth Muscle Actinin (SMA; 1:200; Abcam 5694), Tuj1 (1:200; Covance MMS-435P) and α-fetoprotein (1:200; Dako A 0008). Secondary antibodies used were from Jackson Immunoresearch: DyLight 549-coniugated AffiniPure Goat Anti-Rabbit IgG (1:250; 111-505-303); DyLight 488-coniugated AffiniPure Donkey Anti-mouse IgG (1:250; 715-485-150) and were used according to the manufacturer’s guidelines. Images were taken using an Axiovert 200M microscope (Zeiss).
Amabile G., Di Ruscio A., Müller F., Welner R.S., Yang H., Ebralidze A.K., Zhang H., Levantini E., Qi L., Martinelli G., Brummelkamp T., Le Beau M.M., Figueroa M.E., Bock C, & Tenen D.G. (2015). Dissecting the role of aberrant DNA methylation in human leukemia. Nature communications, 6, 7091.
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2

Pluripotency and Lineage Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown in six-well plates and fixed with 4% paraformaldehyde. The following antibodies were used: OCT4 (1:200; Abcam 19857), NANOG (1:200; Abcam 21624), Tra1-81 (1:200; Abcam 16289), Tra1–60 (1:200; Abcam 16288), Smooth Muscle Actinin (SMA; 1:200; Abcam 5694), Tuj1 (1:200; Covance MMS-435P) and α-fetoprotein (1:200; Dako A 0008). Secondary antibodies used were from Jackson Immunoresearch: DyLight 549-coniugated AffiniPure Goat Anti-Rabbit IgG (1:250; 111-505-303); DyLight 488-coniugated AffiniPure Donkey Anti-mouse IgG (1:250; 715-485-150) and were used according to the manufacturer’s guidelines. Images were taken using an Axiovert 200M microscope (Zeiss).
Amabile G., Di Ruscio A., Müller F., Welner R.S., Yang H., Ebralidze A.K., Zhang H., Levantini E., Qi L., Martinelli G., Brummelkamp T., Le Beau M.M., Figueroa M.E., Bock C, & Tenen D.G. (2015). Dissecting the role of aberrant DNA methylation in human leukemia. Nature communications, 6, 7091.
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