2 n n dimethylamino ethyl methacrylate dmaema

2-(N,N′-Dimethylamino)ethyl methacrylate (DMAEMA, 98%) was obtained from Alfa Aesar. β-Propiolactone (98%) was purchased from J&K Scientific Ltd. 2,2′-Azobis(2-methylpropionitrile) (AIBN), 2-cyanopropan-2-yl benzodithioate and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) were obtained from Sigma Aldrich. HBSS solutions were obtained from Gibco, Lipofectamine 3000 was purchased from Invitrogen. Cy5-siRNA was purchased from Jill Biochemical Reagent Company. Antibodies against human polo-like kinase 1 (Plk1) was obtained from Affinity. Goat anti-rabbit, BCA Protein Assay Kit, Bradford Protein Assay and lysis buffer (RIPA) were purchased from Beyotime (China). siRNAs targeting human Plk1 (siPlk1) (sense strand, 5′-UGAAGAAGAUCACCCUCCUUAdTdT-3′; antisense strand, 5′-UAAGGAGGGUGAUCUUCUUCAdTdT-3′) and scrambled siRNA (siNonsense) (sense strand, 5′-UUCUCCGAACGUGUCACGUdTdT-3′; antisense strand, 5′-ACGUGACACGUUCGGAGAAdTdT-3′) were supplied by Su Zhou Ribo Life Science Co. Ltd. (Suzhou, China). ANNEXIN V-FITC/PI Apoptosis Detection Kit was obtained from Solarbio (Beijing, China). Dulbecco's Modified Eagle Medium (DMEM), penicillin (10 000 U mL⁻¹), streptomycin (10 mg mL⁻¹), trypsin–EDTA and fetal bovine serum (FBS) were purchased from Thermo. All other reagents used were obtained commercially at analytical grade.