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Mouse tnf α elisa max

Manufactured by BioLegend

The Mouse TNF-α ELISA MAX is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kit designed to measure mouse tumor necrosis factor-alpha (TNF-α) levels in biological samples.

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3 protocols using mouse tnf α elisa max

1

Cytokine Quantification by ELISA

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The production of IL-6, TNF-α, IL-12(p40) and IL-10 was measured by commercial assays: mouse IL-6 ELISA MAX™ Standard Set (BioLegend), mouse TNF-α ELISA MAX™ (BioLegend) Standard Set, the BD OptEIA™ Mouse IL-12(p40) ELISA Set (BD Biosciences) or BD OptEIA™ Mouse IL-10 ELISA Set (BD Biosciences). The ELISA assays were conducted according to the protocols of the distributing companies. The TecanGENios Pro plate reader was used to measure the detectable signal. The results were analyzed with the Magellan5 software.
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2

Adipocyte and SVC Cytokine Assays

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The following ELISAs were performed in supernatants from isolated adipocytes and SVCs according to the manufacturer's protocol: MCP-1 (Mouse MCP-1 ELISA MAX, Biolegend, San Diego, CA), TNF-α (Mouse TNF-α ELISA MAX, Biolegend, San Diego, CA), Chemerin (Mouse Chemerin DuoSet ELISA, R&D Systems, Minneapolis, MN), lipocalin-2 (LCN2) (Mouse LCN2/NGAL DuoSet ELISA, R&D Systems, Minneapolis, MN) and soluble receptor for advanced glycation end products (sRAGE) (Mouse sRAGE DuoSet ELISA, R&D Systems, Minneapolis, MN).
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3

Quantifying TNF-α in LPS-Induced Inflammation

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Serum was collected from animals treated in vivo with LPS 1 mg/kg and EP11313 10 or 30 mg/kg. TNF-α concentration was determined by Mouse TNF-α ELISA MAX (Biolegend, San Diego, CA), following manufacturer's protocol. Analysis was performed using Benchmark Plus microplate spectrophotometer at 405 OD.
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