Jem 1010 transmission electron

Manufactured by JEOL

Sourced in United States

The JEM 1010 is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of microscopic samples. The JEM 1010 utilizes an electron beam to illuminate the sample and capture images, enabling users to study the detailed structure and composition of materials at the nanoscale level.

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Lab products found in correlation

Amt imaging system, by Advanced Microscopy Techniques (2 mentions) Cmos temcamf416, by TVIPS (1 mentions) Whatman, by Cytiva (1 mentions)

Spelling variants of this product

JEM-1010 (752 citations) JEM 1010 transmission electron microscope (291 citations) JEM-1010 transmission (16 citations) JEOL 1010 transmission (11 citations) Jem 1010 electron (3 citations)

4 protocols using jem 1010 transmission electron

Transmission Electron Microscopy of MaMAs

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An aliquot (10 μL)
of MaMAs was placed on 100 mesh carbon-coated, Formvar-coated copper
grids pretreated with poly-l-lysine for ∼1 h. For
imaging with negative staining, the samples were negatively stained
with Millipore-filtered aqueous 2% uranyl acetate. The stain was blotted
dry from the grids with a filter paper, and the samples were allowed
to dry. Then, the samples with or without negative staining were examined
in a JEM 1010 transmission electron microscope (JEOL USA, Inc., Peabody,
MA) at an accelerating voltage of 80 kV. Digital images were obtained
using an AMT imaging system (Advanced Microscopy Techniques Corp.,
Danvers, MA).

Kim C.S., Nevozhay D., Aburto R.R., Pehere A., Pang L., Dillard R., Wang Z., Smith C., Mathieu K.B., Zhang M., Hazle J.D., Bast RC J.r, & Sokolov K. (2022). One-Pot, One-Step Synthesis of Drug-Loaded Magnetic Multimicelle Aggregates. Bioconjugate Chemistry, 33(5), 969-981.

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Transmission Electron Microscopy Specimen Preparation

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A 2.5 μL aliquot was applied
onto freshly glow-discharged, carbon-coated 400 mesh copper grids
(Veco). After brief blotting (Whatman #1), the samples were stained
by using a 2% uranyl acetate solution and air-dried. Images were taken
with a JEM1010 transmission electron microscope (JEOL) equipped with
a 4 × 4 K Tietz CMOS TemCamF416 (TVIPS, Gauting, Germany) at
100 kV.

Godoy-Hernandez A., Asseri A.H., Purugganan A.J., Jiko C., de Ram C., Lill H., Pabst M., Mitsuoka K., Gerle C., Bald D, & McMillan D.G. (2023). Rapid and Highly Stable Membrane Reconstitution by LAiR Enables the Study of Physiological Integral Membrane Protein Functions. ACS Central Science, 9(3), 494-507.

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Nanoformulation Morphology and Uptake

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In vitro release and rheology were reported in our previous study [22 (link)]. A JEM 1010 transmission electron microscope (JEOL, USA, Inc., Peabody, MA) was used to observe the morphology of the filament at an accelerating voltage of 80 kV. Digital images were generated and obtained by the AMT Imaging System (Advanced Microscopy Techniques Corp., Danvers, MA). In the uptake study, nano-cur was incubated with LLC cells for 2 h and then observed by TEM.

Wang T., Wang J., Jiang H., Ni M., Zou Y., Chen Y., Wu T., Ding D., Xu H, & Li X. (2022). Targeted regulation of tumor microenvironment through the inhibition of MDSCs by curcumin loaded self-assembled nano-filaments. Materials Today Bio, 15, 100304.

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Ultracentrifugation and TEM of HearNPV Virions

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HearNPV infected culture supernatants from passage 1 to passage 6 were ultracentrifuged at 100,000 g for 1.5 h to obtain the virion pellets of each sample. The re-suspended pellet in fresh medium was applied to carbon coated copper grids for 3 min and stained by Uranyl acetate. More than 50 virion particles from each sample were observed under a JEM-1010 transmission electron microscope (JEOL, Peabody, MA, USA) at an accelerating voltage of 100 kV. The lengths were measured using the electron micrographs of the transmission electron microscope.

Matindoost L., Nielsen L.K, & Reid S. (2015). Intracellular Trafficking of Baculovirus Particles: A Quantitative Study of the HearNPV/HzAM1 Cell and AcMNPV/Sf9 Cell Systems. Viruses, 7(5), 2288-2307.

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