Ceramide/PLGA nanoparticles were fabricated following the previous research30 (link): 25 mg poly lactic-co-glycolic acid (PLGA, Sigma-Aldrich, MO, USA) and 10 mg Ceramide (Doosan, South Korea) were dissolved in 0.5 ml chloroform (Sigma-Aldrich, MO, USA), then mixed with 10 ml 2.5% (w/v) polyvinyl alcohol (Junsei, Japan). The resultant sample was subjected to sonication (Sonictopia, South Korea) for 30 s, after which the chloroform was removed by stirring at 4 °C for 24 h. Next, 0.5 ml of 10 mg/ml chitosan (Sigma-Aldrich, MO, USA) dissolved in 1% acetic acid (Daejung, South Korea) was added to each sample and allowed to react for 2 h to develop a chitosan coating on the nanoparticle.
Polyvinyl alcohol (pva)
Manufactured by Junsei
Sourced in Japan
Polyvinyl alcohol is a synthetic polymer used in various laboratory applications. It is a water-soluble, odorless, and nontoxic material that can be used for a variety of purposes in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Chitosan, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Poly lactic co glycolic acid plga, by Merck Group (1 mentions)
Acetic acid, by Daejung Chemicals (1 mentions)
Chitosan, by Daejung Chemicals (1 mentions)
Glycolide, by Merck Group (1 mentions)
Stannous octoate, by Merck Group (1 mentions)
Live dead assay kit, by Thermo Fisher Scientific (1 mentions)
Huvecs, by Lonza (1 mentions)
Methanol, by Samchun (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate isomer 1 fitc, by Merck Group (1 mentions)
Gelatin type a, by MP Biomedicals (1 mentions)
Fetal bovine serum (fbs), by Capricorn (1 mentions)
Formaldehyde solution, by Fujifilm (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Nih3t3, by Korean Cell Line Bank (1 mentions)
White beeswax, by Samchun (1 mentions)
4 protocols using polyvinyl alcohol (pva)
1
Ceramide/PLGA Nanoparticle Fabrication Protocol
2
Bioactive Polymeric Nanoparticle Synthesis
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Two types of mPEG (molecular weight [MW] 2,000 and 5,000 Da) were purchased from Acros Organics (Gael, Belgium) and Sigma Aldrich (St Louis, MO, USA), respectively. Lactide, glycolide and stannous octoate were purchased from Sigma Aldrich. Before use, mPEG was dried in vacuum to remove moisture, and D,L-Lactide and glycolide were recrystallized from ethyl acetate and stored at −20°C. Diethylenetriamine NONOate (DETA NONOate) was obtained from Acros Organics. Dichloromethane (DCM), toluene, methanol and ethyl acetate were purchased from Samchun Chemical (Seoul, Korea). Poly(vinyl alcohol) was purchased from Junsei Chemical (Tokyo, Japan). HUVECs were purchased from Lonza (Basel, Switzerland). The 3T3 fibroblast, MCF-7 (breast adenocarcinoma), A549 (lung carcinoma) and C6 (mouse brain glial cell) cell lines were purchased from Korea Cell Line Bank (Seoul, Korea). Human EP cells (PCS-600-010) were purchased from ATCC (Manassas, VA, USA). The cell counting kit-8 (CCK-8) was purchased from Dojondo Molecular Technologies Inc. (Rockville, MD, USA). The Live/Dead Assay kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
3
Fabrication of Fluorescent Cell Culture Substrates
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PCL was purchased from
Polysciences (Warminster, PA). Gelatin type A was purchased from MP
Biomedicals (Illkirch, France). PVA was purchased from Junsei (Tokyo,
Japan). GTA 50% aqueous solution was purchased from Daejung Chemicals
& Metals Co., Ltd. (Gyeonggi-do, Republic of Korea). Fluorescein
isothiocyanate isomer I (FITC) and 4′,6-diamidino-2-phenylindole
(DAPI) dihydrochloride were purchased from Sigma-Aldrich (St. Louis,
MO). The formaldehyde solution was purchased from Wako Chemicals (Osaka,
Japan). Triton X-100 was purchased from Yakuri Chemicals Co., Ltd.
(Tokyo, Japan). Alexa Fluor 488 phalloidin was purchased from Invitrogen
(Carlsbad, CA). Dulbecco’s modified Eagle’s medium (DMEM),
phosphate-buffered saline, and streptomycin/penicillin solution were
purchased from Gibco (Grand Island, NY). Fetal bovine serum (FBS)
was purchased from Capricorn (Ebsdorfergrund, Germany). Mouse embryonic
fibroblast cell line NIH3T3 was obtained from the Korean Cell Line
Bank (Seoul, Republic of Korea). WST-1 assay solution (EZ Cytox) was
purchased from DoGenBio Co., Ltd. (Seoul, Republic of Korea).
Polysciences (Warminster, PA). Gelatin type A was purchased from MP
Biomedicals (Illkirch, France). PVA was purchased from Junsei (Tokyo,
Japan). GTA 50% aqueous solution was purchased from Daejung Chemicals
& Metals Co., Ltd. (Gyeonggi-do, Republic of Korea). Fluorescein
isothiocyanate isomer I (FITC) and 4′,6-diamidino-2-phenylindole
(DAPI) dihydrochloride were purchased from Sigma-Aldrich (St. Louis,
MO). The formaldehyde solution was purchased from Wako Chemicals (Osaka,
Japan). Triton X-100 was purchased from Yakuri Chemicals Co., Ltd.
(Tokyo, Japan). Alexa Fluor 488 phalloidin was purchased from Invitrogen
(Carlsbad, CA). Dulbecco’s modified Eagle’s medium (DMEM),
phosphate-buffered saline, and streptomycin/penicillin solution were
purchased from Gibco (Grand Island, NY). Fetal bovine serum (FBS)
was purchased from Capricorn (Ebsdorfergrund, Germany). Mouse embryonic
fibroblast cell line NIH3T3 was obtained from the Korean Cell Line
Bank (Seoul, Republic of Korea). WST-1 assay solution (EZ Cytox) was
purchased from DoGenBio Co., Ltd. (Seoul, Republic of Korea).
4
Synthesis of Polymer Composite Materials
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PVA (Mw = 22,000 Da, CAS Number 9002-89-5) was purchased from Junsei Chemical Co. (Tokyo, Japan). White beeswax (CAS Number 8012-89-3) was purchased from Samchun Pure Chemical Co. (Pyeongtaek, Republic of Korea). The saponification and acid values of the beeswax are 87–104 and 5–22, respectively, and the melting temperature of the beeswax is about 60.0–67.0 °C. PAA (Mw = 5000 Da, CAS Number 9003-01-4) was purchased from FUJIFILM Wako Pure Chemical Co. (Tokyo, Japan). Distilled water (extra pure grade) used in this study was purchased from Duksan Co. (Ansan, Republic of Korea).
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