RPMI1640, fetal bovine serum and trypsase all were bought from Sigma Co.Ltd (USA). RNeasy Mini Kit were purchased from Hilden Co.Ltd (Germany). Arraystar Human circRNAs chip were purchased from Rockville Co. Ltd(USA). TRIzol reagent, MTT, DMSO, crystal violet, PCR reverse transcription kit were bought from Invitrogen Co.Ltd (USA). Annexin V/PI cell apoptosis kits were purchased from Sigma Co.Ltd (USA). Rabbit anti-HIF-1α antibody (1:1000 dilution, catalog number: ab203848), and Goat Anti-Rabbit IgG H&L (HRP) (1:1000, catalog number: ab205718) were purchased from Abcam Co.Ltd (USA).
Trypsase
Manufactured by Merck Group
Sourced in United States
Trypsinase is an enzyme used in cell and tissue culture laboratories. It is a protease that breaks down proteins, facilitating the detachment and disaggregation of adherent cells from cell culture surfaces.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Reverse transcription pcr kit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti hif 1α antibody, by Abcam (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Pepsase, by Merck Group (1 mentions)
Sephadex g 25 resins, by Nanjing Jiancheng (1 mentions)
Succinic anhydride, by Sinopharm (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Cell cycle assay kit, by Merck Group (1 mentions)
Sensiscript reverse transcriptase kit, by Qiagen (1 mentions)
Il 6 elisa kit, by Merck Group (1 mentions)
Platinum quantitative pcr supermix udg, by Thermo Fisher Scientific (1 mentions)
Sybr green, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
4 protocols using trypsase
1
Hypoxia-induced circRNA expression
2
Skipjack Tuna Muscle Bioactivities
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Skipjack tuna (K. pelamis) muscle was provided by Ningbo Today Food Co., Ltd (China). Sephadex G-25 resins and NO assay kit (Nitrate reductase approach, A012-1) were purchased from Nanjing Jiancheng Bioengineering Institute (China). ET-1 ELISA Kit (HM10108) was purchased from Shanghai Qiaodu Biotechnology Co. Ltd. (China).Trypsase (≥20,000 U/g), pepsase (≥500,000 U/g), papain (≥400,000 U/g), ACE (A6778) and N-[3-(2-Furyl) acryloyl]-Phe-Gly-Gly (FAPGG, F713) were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (China). Alcalase (B8360, 2.0 × 105 U/g), 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT; M8180), Penicillin-Streptomycin Liquid (P1400), dimethylsulfoxide (DMSO), fetal bovine serum (FBS; 11011-8611), HEPES buffer (H8090), norepinephrine (NE; IN0530), and Cap (C7510) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (China). ACEi Peptides of (TMAP1-TMAP6) with purity higher than 98% were synthesized in Shanghai Apeptide Co. Ltd. (China).
3
Multifunctional Nanoparticles for Cancer Therapy
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HA (molecular weight [MW]: 3.3×105) was obtained from Freda Biological Technology Co., Ltd (Shandong, People’s Republic of China). CS (MW: 1×106) with a deacetylation degree of 95% was obtained from Jinke Biochemistry Co., Ltd (Zhejiang, People’s Republic of China). Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), ethylene glycol, and succinic anhydride were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, People’s Republic of China). 3-(Aminopropyl) trimethoxysilane, doxorubicin (DOX), folic acid (FA), 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were purchased from Aladdin Reagent Co., Ltd (Shanghai, People’s Republic of China). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), trypsase, penicillin–streptomycin 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and Hoechst33258 were purchased from Sigma-Aldrich Co (St Louis, MO, USA). All the other reagents were of analytical grade. HepG2 cells and L02 cells came from Shanghai Institutes for Biological Sciences of the Chinese Academy of Sciences (Shanghai, People’s Republic of China).
4
HCMV Infection Detection and Cytokine Analysis
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Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), SYBR Green, penicillin, streptomycin, L-glutamine, trypsase, dimethlsulfoxide (DMSO), Triton X-100, phenylmethanesulfonyl fluoride (PMSF), â-mercaptoethanol (â-ME), TNF-α detection kit, IL-6 ELISA kit, and cell cycle assay kit were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-HCMV IE and mouse anti-HCMV UL83 were purchased from Abcam, USA. Secondary antibody goat anti-mouse and Platinum® Quantitative PCR SuperMix-UDG were obtained from Invitrogen, USA. RNeasy Mini Kit and Sensiscript Reverse Transcriptase Kit were purchased from Qiagen (Valencia, CA, USA).
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