Mouse il 4 elisa kits

Arctigenin, hederagenin, and baicalein were purchased from Chemfaces (Hubei, China). 2,4-Dinitrochlorobenzene (DNCB), corticotropin-releasing hormone CRH, substance P (SP), 1-thioglycerol, U0126, SB202190, and SP600125 were obtained from Sigma-Aldrich (St. Louis, MO, USA). High-glucose Dulbecco's modified Eagle's medium (DMEM) was obtained from Welgene Inc. (Gyeongsangbuk, Korea). Iscove's modified Dulbecco's medium (IMDM) was purchased from Merck Millipore (Darmstadt, Germany). Fetal bovine serum (FBS) and antibiotics were obtained from Invitrogen Inc. (Carlsbad, CA, USA). Human IL-6, ICAM-1, VEGF, and mouse IL-13, TNF-α, IFN-γ, IL-12, VEGF, and IgE ELISA kits were purchased from Koma Biotech Inc. (Seoul). Human TARC/CCL17, MDC/CCL22, and mouse IL-4 ELISA kits were obtained from R&D Systems (Minneapolis, MN, USA). Antibodies for phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated c-Jun N-terminal kinase (p-JNK), and phosphorylated p38 (p-p38) were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated anti-β-actin was obtained from Sigma-Aldrich (St. Louis, MO, USA).

DNCB, mite extract, phorbol 12-myristate 13-acetate (PMA), A23187, and carboxyfluorescein succinimidyl ester (CFSE) were purchased from Sigma (St. Louis, MO). Mouse IgG2a enzyme-linked immunosorbent assay (ELISA) kits, anti-mouse CD4 antibody conjugated with fluorescein isothiocyanate (FITC), anti-mouse interferon (IFN)γ antibody conjugated with PerCP cy5.5, and anti-mouse IL-4 antibody conjugated with phycoerythrin were obtained from eBioscience (San Diego, CA). Rabbit anti-mouse keratin5 antibody was purchased from Abcam (Cambridge, MA) and rabbit anti-mouse p65 antibody was from Cell signaling technology (Beverly, MA). Mouse IgE ELISA kits, purified anti-mouse IFNγ, anti-mouse IL-4, and anti-mouse IL-12 antibodies were purchased from BD Bioscience (San Jose, CA). Anti-mouse CD28 antibody, mouse IFNγ ELISA kits, mouse IL-4 ELISA kits, and recombinant human IFNγ and TNFα were purchased from R&D (Minneapolis, MN). Recombinant mouse IL-4 and IL-12 were obtained from Peprotech (Hamburg, Germany). 145-2C11 (mouse anti-CD3; CRL-1975) hybridoma cell line and HaCaT (human keratinocyte) were obtained from the ATCC (Manassas, VA).