Tu212

Manufactured by Thermo Fisher Scientific

Sourced in United States

The TU212 is a laboratory centrifuge designed for general-purpose applications. It provides reliable and efficient sample separation through controlled centrifugation. The core function of the TU212 is to safely and effectively spin down samples in various laboratory workflows.

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Lab products found in correlation

6 protocols using tu212

Macrophage Differentiation and Co-culture Protocol

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Human laryngeal squamous cell lines TU212, TU177, and human mononuclear cell line THP-1 were purchased from the Chinese Academy of Sciences Cell Bank of Type Culture Collection. The cell lines were maintained at 37 °C in a humidified atmosphere of 5% CO2 with RPMI-1640 medium supplemented with 10% fetal calf serum (FBS) with 100 U/ml penicillin G and 100 μg/ml streptomycin sulfate.
To induce differentiation into macrophages, THP-1 cells (1 × 106/ml) were incubated for 24 ~ 48 h with 100 ng/ml PMA (Sigma, MO, USA), and PBS was rinsed three times to produce M0 macrophage. Before exosomes extraction, TU212 cells and TU177 cells were cultured for 48 h with serum without 10% FBS.
THP-1 cells (1 × 106) were planted in the lower chamber with 0.4 mm pores (catalog number140660; Thermo Fisher Scientific, Waltham, MA) in accordance with the Transwell chamber principle. After PMA was added for 24 h ~ 48 h, the cells adhered to the wall, TU212 cells and TU177cells (1 × 105) cells were planted in the insert chamber.

Wang J., Wang N., Zheng Z., Che Y., Suzuki M., Kano S., Lu J., Wang P., Sun Y, & Homma A. (2022). Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma. BMC Cancer, 22, 1208.

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Evaluation of Esculetin and STAT3 Modulators in Laryngeal Cancer

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Human laryngeal cancer cell lines Hep-2, TU-212, and M4e, and human tubular epithelial cell line HK2 were purchased from the American Type Culture Collection (Manassas, VA USA). The Hep-2, TU-212, and HK2 cells were cultured in RPMI Medium 1640 (Thermo Fisher, Waltham, MA, USA), and M4e cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA). Cells were cultured in a 37°C incubator containing 5% CO₂. When growth was in logarithmic phase, cells were seeded onto 96-well plates for further study.
Esculetin was purchased from Sigma-Aldrich (99.99% purity) as 100 mM stock solution, and cisplatin was also purchased from Sigma-Aldrich. STAT3 inhibitor C188-9 was purchased from Selleck Chemicals (Houston, TX, USA) and the STAT3 activator colivelin was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). In an in vitro study, Esc, C188-9, and cisplatin were serially diluted with RPMI Medium 1640 triple and triple. Final working concentrations were 0.0457, 0.1369, 0.4120, 1.229, 3.700, 11.10, and 33.29 μM and the highest working concentration was 100 μM.

Zhang G., Xu Y, & Zhou H.F. (2019). Esculetin Inhibits Proliferation, Invasion, and Migration of Laryngeal Cancer In Vitro and In Vivo by Inhibiting Janus Kinas (JAK)-Signal Transducer and Activator of Transcription-3 (STAT3) Activation. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 7853-7863.

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Cultivation of Highly and Less Malignant Human LSCC Cell Lines

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Human LSCC cell lines, TU212 (highly malignant) and TU686 (less malignant), were obtained from Shanghai Cell Bank, Chinese Academy of Sciences. Both TU212 and TU686 cell lines were routinely cultured in Dulbecco’s modified Eagle medium (DMEM, #11965118; Gibco, New York, USA), supplemented with 10% fetal bovine serum (FBS, #16000; Gibco), 100 µ/ml penicillin, and 100 mg/ml streptomycin (#15140-122; Gibco) in a humidified atmosphere containing 5% CO₂ at 37°C.

Huang C., Wang Z., Zhang K., Dong Y., Zhang A., Lu C, & Liu L. (2019). MicroRNA-107 inhibits proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1 in vitro. Anti-Cancer Drugs, 31(3), 260-271.

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Characterization of Cell Lines for Cancer Research

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HaCaT, TU212, and TU686 cells were purchased from iCell Biotechnology Co., LTD. (Shanghai, China). All three cells had STR testing certificates (Fig. S1). According to Mycoplasma Detection Kit (#G238; abm, Canada), all cell lines were negative for mycoplasma (Fig. S2). TU212 is a laryngeal squamous cell carcinoma cell line and TU686 is a hypopharyngeal carcinoma cell line. TU212 and TU686 cells were cultured in DMEM (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere containing 5% CO₂. Prior to subsequent experiments, cells were treated with MK2206 (5um), a highly selective Akt inhibitor, for 12 h.

Zhu Q., Zhang X., Lu F., Miao S., Zhang C., Liu Z., Gao Z., Qi M., An X., Geng P., Wang S., Ren H., Han F., Zhang R, & Zha D. (2024). RUNX1-BMP2 promotes vasculogenic mimicry in laryngeal squamous cell carcinoma via activation of the PI3K-AKT signaling pathway. Cell Communication and Signaling : CCS, 22, 227.

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Characterization of Head and Neck Cancer Cell Lines

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Human pharyngeal squamous cell carcinoma cell line FaDu (RRID: CVCL_1218), AMC-HN-9 (RRID: CVCL_5967), TU212 (RRID: CVCL_4915), 5-8F (RRID: CVCL_C528) and NP69SV40T (RRID: CVCL_F755) was purchased from cell bank of Chinese Academy of Sciences (shanghai, China). All cells were grown under standard cell culture conditions of 37°C 5% CO 2 and 95% humidity. The FaDu and NP69SV40T cells were cultured in Dulbecco's modi ed Eagle medium (DMEM, Gibco) with 10% fetal bovine serum (FBS, Gibco, USA), 100 IU/ml penicillin and 100µg/ml streptomycin and maintained at low passage number. AMC-HN-9 and 5-8F cells were cultured in 1640 (Gibco, USA). TU212 was cultured in DMEM/F12 (Gibco, USA). All cells were tested for mycoplasma contamination before use. All cell lines were genotyped to establish identity within 6 months of experimentation. Rapamycin was purchased from (Absin, shanghai), A 20 mM solution was prepared in dimethyl sulfoxide and stored at -20℃ for in vitro experiments.

Yang X., Cui L., Liu Z., Li Y., Wu X., Tian R., Jia C., Ren C., Mou Y, & Song X. (2024). TMEM16A inhibits autophagy and promotes the invasion of hypopharyngeal squamous cell carcinoma through mTOR pathway. Carcinogenesis, 45(8).

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Laryngeal Cancer Cell Lines Profiling

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Human laryngeal cancer cell lines, TU212 and M4e, human normal larynx epithelial HBE cells, and human normal liver HHL-5 cells, were purchased from the American Type Culture Collection (ATCC; Manassas, VA USA). The mouse normal larynx epithelial RTE cells, and human laryngeal cancer HEP-2 cells were purchased from the Nanjing KeyGen Biotech, Co., Ltd. (Nanjing, China). TU212, HEP-2 and RTE cells were routinely cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA), containing 10% fetal bovine serum (FBS; Gibco), 1% penicillin/streptomycin. The cell lines M4e, HBE and HHL-5 were cultured in Dulbeccos modified Eagles medium (DMEM; Gibco) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. All cells were kept in a humidified atmosphere with 5% CO₂ and 95% humidity at 37°C in an incubator. Galangin (>98% purity), purchased from the Hangzhou DayangChem, Co., Ltd., (Hangzhou, China) was used for the treatment of human laryngeal cancer dissolved in dimethyl sulfoxide (DMSO) and then stored at −20°C for experimental treatment use. The final DMSO concentration in cells is <0.1% (v/v) in each treatment.

Wang H.X, & Tang C. (2017). Galangin suppresses human laryngeal carcinoma via modulation of caspase-3 and AKT signaling pathways. Oncology Reports, 38(2), 703-714.

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