Quantikine hs human il 6 kit

Plasma CK protein activity levels were measured as previously described [5 (link)]. Plasma HSP70 concentrations were assayed by using the HSP70 High Sensitivity EIA kit (Catalog # EKS-715, ENZO life Science, Ann Arbor, MI, USA) as per package protocol. Detection range of the HSP70 assay was 0.20–12.50 ng/mL; the intra- and interassay coefficients of variation were <10 and <6%, respectively. Plasma IL-6 protein (IL-6) concentrations were assayed using the Quantikine HS Human IL-6 kit (Catalog # SS600B, R&D Systems, Minneapolis, MN, USA) as per package protocol. Detection range of the IL-6 assay was 0.156 to 10 pg/mL; the intra- and interassay coefficients of variation were <10 and <7%, respectively. Any sample above the detection limit for the HSP70 or IL-6 assay was rediluted to be within the detection limit and reassayed as per assay instructions. All HSP70 and IL-6 assays had the same duplicate control sample to validate interassay results.

Serum IL-6 levels were measured using a commercial quantitative sandwich enzyme-linked immunosorbent assay according to the kit procedure (Quantikine HS Human IL-6 kit; R & D Systems, Minneapolis, MN). The assay range was 0.156–10 pg/ml; the minimum detectable dose was 0.039 pg/ml and lower levels were considered undetectable.