3 2 pyridyl 5 6 diphenyl 1 2 4 triazine p p disulfonic acid monosodium salt hydrate ferrozine

Manufactured by Merck Group

Sourced in United States

3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) is a chemical compound used in analytical chemistry. It is a sensitive chromogenic reagent for the colorimetric determination of iron in various samples.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Ferrozine (190 citations) P’-disulfonic acid monosodium salt hydrate (2 citations)

9 protocols using 3 2 pyridyl 5 6 diphenyl 1 2 4 triazine p p disulfonic acid monosodium salt hydrate ferrozine

Iron Chelation Activity Evaluation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To evaluate the iron chelation activity by FMRPs, 100 μL of FMRPs (20 mg/mL), 600 μL of distilled water, and 100 μL of 0.2 mM iron (II) chloride tetrahydrate (FeCl₂·4H₂O, SAMCHUN chemical Co. Ltd., Seoul, Korea) were mixed, and the mixture reacted for 30 sec at room temperature. Subsequently, 200 μL of 0.1 mM Ferrozine (3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p’-disulfonic acid monosodium salt hydrate; Sigma Aldrich, St. Louis, MO, USA) were suspended in the mixture, and the samples reacted for 10 min at room temperature. Thereafter, turbidity of the samples was measured at 562 nm with a UV-spectrophotometer (OPTIZEN 2120UV, Mecasys Co., Ltd., Daejeon, Korea), and iron chelation activity was calculated as follows;
Absorbance of blank solution was measured with distilled water instead of with samples [15 (link)].

Kim Y., Kim S., Lee S., Ha J., Lee J., Choi Y., Oh H., Lee Y., Oh N.S., Yoon Y, & Lee H. (2021). Antimicrobial activity of fermented Maillard reaction products, novel milk-derived material, made by whey protein and Lactobacillus rhamnosus and Lactobacillus gasseri on Clostridium perfringens. Animal Bioscience, 34(9), 1525-1531.

+ Open protocol

+ Expand

Preparation of High-Purity Fe(II) Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ultrapure water (pure water,
resistivity ≥18.2 MΩ cm) was freshly obtained from a
pure water system (Millipore, USA) and used for the preparation of
all solutions. Ferrozine (3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium
salt hydrate) was obtained from Sigma-Aldrich (USA). Bondesil-C18
particles (40 μm) were obtained from Agilent Technologies (USA).
All other chemicals used were of analytical or reagent grade and supplied
by the Sinopharm Chemical Reagent (China).
A stock solution
of 10.0 mmol L^–1 Fe(II) was prepared by dissolving
ferrous ammonium sulfate in 1 mol L^–1 hydrochloric
acid and stored at 4 °C while not in use. Working solutions of
Fe(II) were freshly prepared by diluting the stock solution with pure
water and adding solid ascorbic acid to a concentration of about 0.1
mol L^–1 to maintain the redox species of Fe(II).

Ma M., Wang H., Xu J., Huang Y., Yuan D., Zhang X, & Song Q. (2020). An In Situ Analyzer for Two-Dimensional Fe(II) Distribution in Sediment Pore Water Based on Ferrozine Coloration and Computer Imaging Densitometry. ACS Omega, 5(49), 31551-31558.

+ Open protocol

+ Expand

Enzymatic Extraction and Antioxidant Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protamex and Alcalase L 2.4 FG were purchased from Novozymes (Bagsvaerd, Denmark). Sodium dihydrogen phosphate (NaH₂PO₄), disodium hydrogen phosphate (Na₂HPO₄), extran neutral MA02, (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic) acid [ABTS]), and potassium persulfate (K₂S₂O₈) were purchased from Merck (Darmstadt, Germany). Iron (II) sulfate (FeSO₄) and 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (Ferrozine) were purchased from Sigma-Aldrich (St. Louis, MO). 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), sodium ascorbate, hydrochloric acid, methyl linoleate, and tween-20 were purchased from Sigma-Aldrich (Steinheim, Germany). Hemin was purchased from Acros Organics (Geel, Belgium). Ethanol (99.9%) was purchased from Chemethyl A/S (Køge, Denmark), and sodium hydroxide was purchased from Sigma-Aldrich (Renningen, Germany).

Damgaard T.D., Otte J.A., Meinert L., Jensen K, & Lametsch R. (2014). Antioxidant capacity of hydrolyzed porcine tissues. Food Science & Nutrition, 2(3), 282-288.

+ Open protocol

+ Expand

Mungbean Meal Antioxidant Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mungbean meal and bromelain was provided from Sittinun Co., Ltd., Thailand and K-Much-Industry Co., Ltd. (Bangkok, Thailand). Mungbean meal was defatted using hexane based on a previously described method (Sonklin, Laohakunjit & Kerdchoechuen, 2011 (link)). All chemical reagents were of analytical grade. 2,2-diphenyl-1-picrylhydrazyl (DPPH), glutathione reduced (GSH), 1-10-Phenanthroline, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) and pyrogallol were purchased from Sigma Aldrich (MO, USA). All other reagents were purchased from Fisher Scientific (Hampton, NH, USA) except stated otherwise.

Sonklin C., Laohakunjit N, & Kerdchoechuen O. (2018). Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates. PeerJ, 6, e5337.

+ Open protocol

+ Expand

Quantification of Antioxidant Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemicals, including 2,2-azino-bis(3-ethylbenzothiazoline-6-sul-phonic acid) diammonium salt (ABTS), 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine), ferrous chloride, bovine serum albumin, aprotinin, Vitamin B12, hippuril-L-histidyl-Lleucine (HHL), and norleucine were purchased from Sigma-Aldrich. Potassium persulphate and glycine were obtained from Panreac. 2,4,6-tri (2-pyridyl)-s-triazine (TPTZ) was obtained from Fluka. All other reagents were of analytical grade.

Sepúlveda C.T., Zapata J.E., Martínez-Álvarez O., Alemán A., Montero M.P, & Gómez-Guillén M.C. (2021). The preferential use of a soy-rapeseed lecithin blend for the liposomal encapsulation of a tilapia viscera hydrolysate. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 139.

+ Open protocol

+ Expand

Antioxidant Assay Protocol for Bioactives

Check if the same lab product or an alternative is used in the 5 most similar protocols

Absolute and deuterated methanol, tetramethylsilane (TMS), ascorbic acid, Folin-Ciocalteu’s phenol reagent and gallic acid were procured from Merck (Darmstadt, Germany). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) reagent, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) reagent, 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p, p’-disulfonic acid monosodium salt hydrate (Ferrozine), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and quercetin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cell culture chemicals and media were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Ng K.S., Tan S.A., Bok C.Y., Loh K.E., Ismail I.S., Yue C.S, & Loke C.F. (2022). Metabolomic Approach for Rapid Identification of Antioxidants in Clinacanthus nutans Leaves with Liver Protective Potential. Molecules, 27(12), 3650.

+ Open protocol

+ Expand

Enzymatic Assays for Antioxidant Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

α-Amylase from hog pancreas (CAS No. 9000-90-2), porcine bile extract (CAS No. 8008-63-7), lipase from porcine pancreas (CAS No. 9001-62-1), mucin from porcine stomach Type II (CAS No. 84082-64-4), pepsin from porcine gastric mucosa (CAS No. 9001-75-6), crystalline uric acid (≥99%; CAS No. 69-93-2), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt (CAS No. 30931-67-0), 2,2-diphenyl-1-picrylhudrazyl (DPPH; CAS No. 1898-66-4), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine), iron (II) chloride (CAS No. 7758-94-3), and iron (III) chloride (CAS No. 7705-08-0) were purchased from Sigma-Aldrich (St. Louis, MA, USA). EDTA-2Na dihydrate (CAS No. 6381-92-6), trichloroacetic acid (TCA; CAS No. 76-03-9), sodium phosphate dibasic anhydrous (CAS No. 7558-79-4), and sodium phosphate monobasic anhydrous (CAS No. 7558-80-7) were purchased from Daejung Chemicals (Siheung, Korea). Potassium hexacyanoferrate (III) (CAS No. 13746-66-2) was purchased from Kanto Chemical (Tokyo, Japan) and 2,6-di-tert-butyl-4-methylphenol (99.0%) was purchased from Samchun Pure Chemical (Seoul, Korea).

Kim J.H., Lee D.Y., Lee S.Y., Mariano E J.r., Jeong J.W., Yun S.H., Lee J., Park J., Choi Y., Han D., Kim J.S., Jo C, & Hur S.J. (2024). Study on the Digestion-Induced Changes in the Characteristics and Bioactivity of Korean Native and Overseas Cattle-Derived Peptides. Food Science of Animal Resources, 44(3), 551-569.

+ Open protocol

+ Expand

Microalgae Cultivation Media Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Macronutrients used were NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄·2H₂O (Honeywell International Inc., Charlotte, NC, USA), while the trace elements used for media preparation were Na₂EDTA (Sigma-Aldrich, St. Louis, MO, USA), FeCl₃·6H₂O (Acros Organics, Geel, Belgium), CuSO₄·5H₂O (Sigma-Aldrich), ZnSO₄·7H₂O (Sigma-Aldrich), CoCl₂·6H₂O (Thermo Fisher Scientific, Pittsburg, PA, USA), MnCl₂·4H₂O (Acros Organics), Na₂MoO₄·2H₂O (Chem-Lab NV, Zedelgem, Belgium). Cyanocobalamin, Thiamine HCl and Biotin were purchased from Sigma-Aldrich.
Chemicals used for analysis were ammonium bicarbonate (Sigma-Aldrich), Folin–Ciocalteu reagent (Sigma-Aldrich), N,N′-dimethylformamide (DMF) (Honeywell), chloroform HPLC grade (Honeywell), mercury (III) oxide red (Sigma-Aldrich), 2,4,6-tri(2–pyridyl)–s–triazine (TPTZ) (Alfa Aesar, Ward Hill, MA, USA), water for analysis (Carlo Erba, Cornaredo, Milan, Italy), methanol HPLC grade (Thermo Fischer Scientific), sodium acetate trihydrate (Merck), acetic acid (PENTA), hydrochloric acid (HCl) (PENTA), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH∙) (Sigma-Aldrich), iron (II) chloride anhydrous (Acros organics), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) (Sigma-Aldrich), EDTA-Na₂, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (Acros organics).

Andriopoulos V., Gkioni M.D., Koutra E., Mastropetros S.G., Lamari F.N., Hatziantoniou S, & Kornaros M. (2022). Total Phenolic Content, Biomass Composition, and Antioxidant Activity of Selected Marine Microalgal Species with Potential as Aquaculture Feed. Antioxidants, 11(7), 1320.

+ Open protocol

+ Expand

Quantification of Antioxidant Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rincón C.T.S., Zapata J.E., Martínez‐Álvarez Ó., Alemán A., Montero P., & Gómez‐Guillén M. (2021). The preferential use of a soy-rapeseed lecithin blend for the liposomal encapsulation of a tilapia viscera hydrolysate. LWT, 139, 110530-110530.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!