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2 protocols using norbuprenorphine

1

Certified Reference Standards for Drugs

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Certified reference standards of 6-monoacetylmorphine, alprazolam, amphetamine, buprenorphine, benzoylecgonine, carisoprodol, chlordiazepoxide, clonazepam, cocaine, codeine, cotinine, dihydrocodeine, diazepam, EDDP, fentanyl, flurazepam, hydrocodone, hydromorphone, lorazepam, MDA, MDEA, MDMA, methamphetamine, meperidine, morphine, methylphenidate, methadone, naloxone, norbuprenorphine, nicotine, nordiazepam, norfentanyl, noroxycodone, alpha-hydroxyalprazolam, oxazepam, oxycodone, oxymorphone, tapentadol, temazepam, ll-nor-9-Carboxy-Δ9-THC, and cis-tramadol were purchased, each at a concentration of 1 mg/mL, from Cerilliant Corporation (Round Rock, TX). Isotopically labeled internal standards (IS) morphine-D3, codeine- D6, hydrocodone-D3, 6-monoacetylmorphine-D6, hydromorphone-D6, oxymorphone-D3, noroxycodone-D3, and naloxone-D5 were purchased, each at a concentration of 100 μg/mL, from Cerilliant Corporation (Round Rock, TX). Drug-free urine was obtained from Bio-Rad Laboratories (Irvine, CA). High-performance liquid chromatography (HPLC) grade water, methanol, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA) and ammonium formate was purchased from Sigma Aldrich (St. Louis, MO).
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2

Quantitative Analysis of Opioids and Antipsychotics

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Surine DOA (synthetic urine) was purchased from Dyna-Tek Industries (Lenexa, KS). Buprenorphine, norBuprenorphine, naloxone and their respective deuterated internal standards were obtained from Cerilliant (Austin, TX). For quetiapine metabolite characterization experiments, quetiapine, 7-hydroxyquetiapine and quetiapine acid were also acquired from Cerilliant, and quetiapine sulfoxide was purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). HPLC-grade acetonitrile, methanol and ammonium hydroxide (ACS grade) were obtained from Thermo Fisher (Waltham, MA). Formic acid (98%, ACS grade) was purchased from EMD Millipore (Billerica, MA), and phosphoric acid (ACS grade) was purchased from Sigma-Aldrich (St. Louis, MO). Purified water was supplied by an in-house MilliQ system (EMD Millipore). IMCSzyme recombinant β-glucuronidase and Rapid Hydrolysis Buffer (pH 7.2–7.8), used for enzymatic hydrolysis in all laboratories, were purchased from IMCS (Integrated Micro-Chromatography Systems, Irmo, SC).
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