Phenotypic analyses were performed in FACSCalibur and FACSCanto II (BD Biosciences) flow cytometers using the following mAbs: PE-labeled anti-CD1a, anti-CD34, anti-CD127, and anti-TCRαβ (Beckman Coulter); anti-CD3, anti-CD13, anti-CD44, anti-CD45RA, anti-CD123, anti-CD135, anti-HLA-DR, and anti-Notch4 (BD Biosciences); anti-CD116 (Immunotech); anti-Notch1 and anti-Notch3 (BioLegend); PC5-labeled anti-CD7 (Caltag); anti-CD4, anti-CD5, anti-CD13, anti-CD33, and anti-CD34 (Beckman Coulter); BB515-labeled anti-CD115 (BD Biosciences); FITC-labeled anti-BDCA1 and anti-BDCA2 (Miltenyi Biotec); anti-CD11c (Life Technologies) and anti-CD5, anti-CD7, anti-CD8, anti-CD14, and anti-CD44 (BD Biosciences); V450-labeled anti-CD45; and APC-labeled anti-CD34 (Beckman Coulter) and anti-CD117 (BD Biosciences). Irrelevant isotype-matched Igs (Caltag) were used as controls. Staining with biotin-coupled Annexin V (Roche) plus streptavidin-PE (Invitrogen) and 7-AAD (BD Biosciences) was used for apoptosis analysis. Cell proliferation was quantified by flow cytometry using CFSE labeling, following the manufacturer’s instructions (BD Biosciences).
Anti cd135
Manufactured by BD
Sourced in United States
Anti-CD135 is a monoclonal antibody that binds to the CD135 (also known as FLT3 or Fms-like tyrosine kinase 3) antigen. CD135 is a receptor tyrosine kinase that plays a role in the regulation of hematopoiesis. The antibody can be used for the identification and characterization of CD135-expressing cells in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (2 mentions)
Anti hla dr, by BD (1 mentions)
Anti cd11c, by Thermo Fisher Scientific (1 mentions)
Anti cd117, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
7 aad, by BD (1 mentions)
Anti cd150, by BD (1 mentions)
Anti cd8, by BD (1 mentions)
Anti sca1, by BD (1 mentions)
Anti ter119, by BD (1 mentions)
Anti gr 1, by BD (1 mentions)
Anti b220, by BD (1 mentions)
Anti cd16 32, by BD (1 mentions)
Anti thy1, by BD (1 mentions)
Anti c kit, by BD (1 mentions)
Anti mac 1, by BD (1 mentions)
Anti cd4, by BD (1 mentions)
Anti cd34, by BD (1 mentions)
2 protocols using anti cd135
1
Multiparameter Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Analysis of Hematopoietic Stem and Progenitor Cells
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Cells were stained with FITC-, PE-, Biotin-, APC-, or PE-Cy7-conjugated anti-Ly5.1, anti-Ly5.2, anti-Gr1, anti-Mac1, anti-B220, anti-Thy1.2, anti-Ter119, anti-Sca1, anti-c-kit, anti-CD4, anti-CD8, anti-CD16/32, anti-CD34, anti-CD135, and anti-CD150 antibodies (BD Biosciences, San Jose, CA, USA or eBioscience, San Diego, CA, USA). Detection of HSPCs was performed on a BD FACSCanto™ II (BD Biosciences). The surface marker phenotypes of LSK, LT-HSC (long-term HSC), ST-HSC (short-term HSC), MPP (multipotent progenitor), CMP (common myeloid progenitors), GMP (granulocyte/macrophage progenitors), and MEP (megakaryocyte erythrocyte progenitor) are listed in Supplementary Table 4 .
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