L type wako tg m or nefa hf

Serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-CHO), low-density lipoprotein-cholesterol (LDL-CHO), and free fatty acid (FFA) were measured by Oriental Yeast (Tokyo, Japan) using a Hitachi 7180 Auto Analyzer. AST and ALT were measured by the Japan Society of Clinical Chemistry transferable method using an L type Wako AST or ALT-J2 kit (Fujifilm, Tokyo, Japan), TG and FFA were measured by an enzymatic method using L type Wako TG-M or NEFA HF (Fujifilm), and HDL or LDL-CHO were measured by a direct method using CHOLESTEST N HDL or LDL (Sekisui Medical, Tokyo Japan). Glutathione (GSH) and glutathione disulfide (GSSG) levels in the liver tissue were measured using a GSSG/GSH Quantification Kit (Fujifilm).

Serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-CHO), low-density lipoprotein cholesterol (LDL-CHO), and free fatty acids (FFAs) were measured with Oriental Yeast (Tokyo, Japan) using a Hitachi 7180 Auto Analyzer. AST and ALT were measured with the JSCC transferable method using L type Wako AST or ALT-J2 kit (FUJIFILM, Tokyo, Japan). TGs and FFAs were measured with enzymatic methods using L-type Wako TG-M or NEFA HF (FUJIFILM, Tokyo, Japan). HDL-CHO and LDL-CHO were measured with direct methods using CHOLESTEST N LDL or HDL (SEKISUI MEDICAL, Tokyo, Japan). Glutathione (GSH and GSSG) concentrations and superoxide dismutase (SOD) activity in the liver tissue specimens were measured using a GSSG/GSH Quantification Kit (FUJIFILM, Tokyo, Japan) and SOD Assay kit-WST (FUJIFILM, Tokyo, Japan). Malondialdehyde (MDA) concentrations in liver tissue specimens were measured using a TBARS assay kit (Cayman, Chemical, Ann Arbor, MI, USA).