IgGs utilized were SILu (Sigma MSQC4) and anti-BNP (ICL RBNP-65A-Z). All other proteins were purchased from Sigma-Aldrich: bovine serum albumin (BSA; A7906), human haptoglobin (H3536), bovine carbonic anhydrase (C2624), bovine myoglobin (M5696), bovine α-lactalbumin (L5385), and bovine ubiquitin (U6253). Peptides utilized were a custom peptide (H-GLFYVDFLSQDKV-SIALSSHWINPR-OH, 2894.29 Da; Global Peptide), fibrinopeptide B (1570.6 Da; Sigma F3261), and Z-Leu-Leu-Leu-al (475.62 Da; Sigma C2211). Detergents were purchased from Sigma-Aldrich: CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate hydrate; C3023) and Triton X-100 (X-100). Chromatography solvents were purchased from Fisher: water (Optima LC/MS grade; W64), acetonitrile (Optima LC/MS grade; A9554), and formic acid (Pierce LC/MS grade; PI28905).
Fibrinopeptide b
Manufactured by Merck Group
Fibrinopeptide B is a lab equipment product. It is a protein fragment that is released during the conversion of fibrinogen to fibrin, which is a key step in the blood clotting process. The core function of Fibrinopeptide B is to facilitate the monitoring and analysis of this blood clotting mechanism in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100 x100, by Merck Group (1 mentions)
Z leu leu leu al, by Merck Group (1 mentions)
Bovine ubiquitin, by Merck Group (1 mentions)
Voyager de strtm maldi tof, by Thermo Fisher Scientific (1 mentions)
4700 calibration standard kit, by Thermo Fisher Scientific (1 mentions)
4800 maldi tof tof mass spectrometer, by Thermo Fisher Scientific (1 mentions)
2 protocols using fibrinopeptide b
1
Antibody and Protein Characterization Protocol
2
MALDI-TOF Mass Spectrometry Protocol
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MALDI-TOF spectra were recorded by either a Voyager DE-STRTM MALDI–TOF or a 4800 MALDI-TOF/TOF mass spectrometer (Applied Biosystems, Darmstadt, Germany) with MALDI-TOF/TOF spectra acquired with the latter instrument. The 4700 Calibration standard kit (Applied Biosystems) was used for calibrating the MS mode and fibrinopeptide B (Sigma) was used for calibrating the MS/MS mode. The collision energy for MS/MS was set to 1 kV, and the collision gas was argon. 2, 5-Dihydroxybenzoic acid and 3,4-diaminobenzophenone were used as matrix. Permethylated samples were dissolved in methanol (10 μL) and the solution premixed with matrix (20 mg/mL) with a ratio of 1:1 (v/v) with the mixture (1 μL) being spotted on the plate.
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