Cd49b macs

Manufactured by Miltenyi Biotec

The CD49b MACS is a cell separation reagent designed for the isolation of CD49b-positive cells from various sample types. It uses magnetic beads coated with antibodies specific to the CD49b cell surface marker, allowing for the separation of target cells from the sample using a magnetic field.

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Lab products found in correlation

Magnisort mouse nk cell enrichment kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using cd49b macs

Mouse NK Cell Isolation Protocol

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Mouse NK cells were isolated from fresh spleens of wild-type (WT) or GPR116^−/− mice on C57BL/6 background. NK cells were purified with MagniSort Mouse NK cell Enrichment Kit (Invitrogen), followed by CD49b MACS (Miltenyi). The cell population was highly selected for NK cells with a purity of > 99%.

Guo D., Jin C., Gao Y., Lin H., Zhang L., Zhou Y., Yao J., Duan Y., Ren Y., Hui X., Ge Y., Yang R, & Jiang W. (2023). GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint. Cell & Bioscience, 13, 51.

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Isolation of Murine NK Cells

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Mouse NK cells were isolated from fresh BALB/c, C57BL/6, or C57BL/6 Sirpa⁻^/⁻ spleens 18 h after poly I:C injection (100 µg i.p.; Sigma-Aldrich). After red cell lysis, NK cells were purified with MagniSort Mouse NK cell Enrichment Kit (Invitrogen), followed by CD49b MACS (Miltenyi). This cell population was highly selected for NK cells with a purity of >99%.

Deuse T., Hu X., Agbor-Enoh S., Jang M.K., Alawi M., Saygi C., Gravina A., Tediashvili G., Nguyen V.Q., Liu Y., Valantine H., Lanier L.L, & Schrepfer S. (2021). The SIRPα–CD47 immune checkpoint in NK cells. The Journal of Experimental Medicine, 218(3), e20200839.

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