Mouse anti β actin bs6007m

The harvested cells were lysed with RIPA Lysis Buffer (Strong) plus protease inhibitor cocktail. The supernatant was collected after high-speed centrifugation and was stored for western blotting. The western blotting was carried out according to the methods routinely used. The islets were lysed with RIPA Lysis Buffer (Strong) plus protease inhibitor cocktail. The samples were obtained according to the manufacture’s instruction and applied for western blotting.
The antibodies used in the experiment were as follows: cleaved caspase-3 Antibody (9661) and rabbit anti-pJNK antibody (4668) were purchased from CST; mouse anti-GAPDH (10494-1-AP), rabbit anti-MKP2 antibody (10739-1-AP), and rabbit anti-MKP7 antibody (14237-1-AP) were derived from Proteintech Group (Wuhan, China); rabbit anti-NR4A1 antibody (ab13851 and DF7850) was purchased from Abcam and Affinity; mouse anti-β-Actin (BS6007M) and rabbit anti-tubulin α (BS1699) were purchased from Bioworld Technology.

BA was obtained from Xi’an Kailai Co, Ltd.; Fas was purchased from Apex Bio Technology LLC; Ami and LPS were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, USA). The bicinchoninic acid assay (BCA) kit was procured from Beyotime Institute of Biotechnology Co., Ltd. IL-1β, TNF-α, and IL-6 enzyme-linked immunosorbent assay (ELISA) kits were supplied by Nasheng Biological Technology Co., Ltd. Rabbit-ROCK2 antibody (20248-1-ap), rabbit-calpain1 antibody (10538-1-ap), and rabbit-caspase-3 antibody (19677-1-ap) were supplied by Proteintech Group, Inc. Rabbit-NHE1 antibody (DF9933) was supplied by Affinity. Rabbit-Bcl-2 (Ab32124) antibody was supplied by Abcam plc. Mouse-Bax antibody (Sc-20067) was supplied by Santa Cruz Biotechnology, Inc. Mouse-anti-β-actin (BS6007M) was supplied by Bioworld Technology, Inc.