LoxP mice were obtained from RIKEN BioResource Research Center (Stock Number: RBRC06462, Strain Name: C57BL/6-Prkcd, 3-1-1 Koyadai, Tsukuba, Ibaraki, Japan). CTSK-Cre mice were kindly provided by Professor Jiake Xu from School of Biomedical Sciences, The University of Western Australia. Mice with an OC-cKO of the PKC-δ (PKC-δ cKO) were generated by crossing mice heterozygous for a floxed exon 7 PKC-δ allele (PKC-δ (ex7)flox/wt) with CTSK-Cre mice heterozygously carrying a cyclization recombinase of which the expression is controlled by the CTSK promoter (CTSK-Cre+PKC-δflox/wt). Offspring were genotyped and the presence of the CTSK-Cre transgene was determined on genomic DNA (gDNA) via PCR with primer sequences presented in Supplementary Table S1 . In all the experiments described below, we analyzed CTSK-Cre+PKC-δflox/flox mice that lack PKC-δ in OCs, and CTSK-Cre–PKC-δflox/flox littermates as controls.
Loxp mice
Manufactured by RIKEN BioResource Center
Sourced in Japan
LoxP mice are a type of genetically modified mouse strain. The core function of LoxP mice is to allow for the targeted deletion or modification of specific genes in a controlled and tissue-specific manner. The LoxP (locus of X-over P1) sequences are inserted into the mouse genome, enabling the use of the Cre-loxP recombination system to manipulate gene expression.
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2 protocols using loxp mice
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Osteoclast-Specific PKC-δ Knockout Mice
2
Generation of B-cell-specific PKC-δ knockout mice
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LoxP mice were purchased from RIKEN BioResource Research Center (Stock Number: RBRC06462, Strain Name: C57BL/6-Prkcd, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan), and CD19-Cre mice were purchased from The Jackson Laboratory (Stock Number: 006785, Strain Name: B6.129P2(C)-CD19tm1(cre)Cgn/J, Bar Harbor, ME, USA). Mice with B-cell-selective knockout of the PKC-δ (CD19-Cre+ PKC-δflox/flox) were generated by crossing mice heterozygous for a floxed exon 7 PKC-δ allele (PKC-δ(ex7)flox/+) with heterozygous mice carrying a cyclization recombinase of which the expression is controlled by the CD19 promoter (CD19-Cre+ PKC-δflox/+). Offspring mice were weaned at 3 weeks of age and genotyped by PCR, the presence of the CD19-Cre transgene was determined on genomic DNA via PCR with forward primer: 5′-ATTTGCCTGCATTACCGGTC-3′ and reverse primer: 5′-ATCAACGTTTTCTTTTCGG-3′ resulting in a 377 bp fragment. CD19-Cre-PKC-δflox/flox littermate mice were used as control.
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