Q tof ms spectrometer

The reaction products were dried under nitrogen gas and then were dissolved in methanol for HPLC analysis. The processed samples (10 μl) were subjected to an Agilent 1290 LC System which was coupled to a multiple-wavelength diode array detector and equipped with a ZORBAX SB-C18 column (4.6 × 250 mm, 5 μm) for analysis. Separation column was maintained at 30°C with a flow rate of 1.0 mL/min. The mobile phase consisted of solvents A (0.1% ammonium acetate in double distilled water) and B (methanol). Gradient elution was done for 20-min and the conditions were as follows: 0–5 min, 5–10% B linear; 5–15 min, 10–60% B linear; 15–20 min, 60–90% B linear. Elution of compounds was monitored at 333 nm (p-coumaroyl-CoA and o-coumaroyl-CoA), 346 nm (caffeoyl-CoA, feruloyl-CoA, and isoferuloyl-CoA) and 310 nm (trans-cinnamoyl-CoA).
All reaction products were identified by LC-MS, using the above conditions. The HPLC system was coupled to Q-TOF MS spectrometer (Agilent Technologies, Santa Clara, CA, USA), equipped with an electrospray source in positive ion mode. The conditions of ESI source were as follows: drying gas (N₂) flow rate, 8.0 mL/min; nebulizer, 241 kPa (35 psig); fragmentor voltage, 150 V; collision energy, 30 eV; octopole radio frequency, 250 V and skimmer voltage, 60 V. In addition, the capillary voltage was set at 4.0 kV and the desolvation gas temperature was 325°C.

The chemical constituents contained in the extract were carried out following the work of Chansriniyom et al. [30 (link)]. Characterization was performed by Q-TOF mass spectrometer (Agilent 6540, Singapore) with a column ZORBAX Eclipse Plus C18 (4.6 mmx100 mm, 3.5 μm). The mass spectrometric analysis was performed using an Agilent 6540 Q-TOF-MS spectrometer equipped with an electrospray ionization (ESI) source and Agilent dual nebulizer operating in a negative ionization mode. To identify phytonutrients, peak retention time, and mass data, their fragmented ions were compared to those of registered compounds on two databases, Human Metabolome Database (HMBD) and METLIN Metabolomics Database and Library (Agilent technology).