PEG-200 solution was prepared by dissolving polyethylene Glycol 200 (PEG-200, Cat. No.: ESL3444, FUJIFILM Wako Pure Chemical Co., Osaka, Japan) in MillQ. Dasatinib (Cat. No.: 11498, Cayman Chemical Co., Ann Arbor, MI, USA) and quercetin (Cat. No.: sc-206089A, SCB Santa Cruz Biotechnology Inc., Dallas, TX, USA) were dissolved in the above-prepared PEG-200 solution and mixed thoroughly to make senolytics. Each rat in the post-stress group was orally administered with senolytics at 6.67 mg·kg-1 (D) and 66.7 mg·kg-1 (Q) on days 1 and 7 after L-loop installation, respectively.
Dasatinib
Manufactured by Fujifilm
Sourced in Japan
Dasatinib is a laboratory equipment product manufactured by Fujifilm. It is a multi-target kinase inhibitor used in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dasatinib
1
Senolytic Therapy Optimization in Rats
2
Dasatinib Treatment for Leukemic Engraftment
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After substantial engraftment of leukemic cells was confirmed by BM aspiration, mice (n = 2 per group) were treated with dasatinib (LC laboratories, Woburn, MA) or vehicle. dasatinib was formulated in 0.5% (w/v) methylcellulose solution (Wako, Japan) and delivered orally at 35 mg/kg for 5 days (Monday to Friday) on/2 days off. Leukemic cell chimerism was monitored by flow cytometry analysis of BM aspirates, as described above. Once leukemic cell chimerism in BM reached <5%, the dasatinib dose was reduced to 20 mg/kg. After chimerism had recovered to >90%, mice were sacrificed by cervical dislocation, the femur, tibia, ilium, and brain were removed and mechanically dispersed, and leukemic cells were then isolated by Ficoll–Hypaque density gradient centrifugation and used for FISH analysis.
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