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High performance liquid chromatography grade chloroform

Manufactured by Fujifilm
Sourced in Japan

High-performance liquid chromatography-grade chloroform is a laboratory solvent used in various analytical and separation techniques. It is a clear, colorless liquid with a distinct, characteristic odor. This high-purity solvent is designed for use in high-performance liquid chromatography (HPLC) applications, where its consistent quality and performance are essential.

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2 protocols using high performance liquid chromatography grade chloroform

1

Lipid Extraction and Quantification

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LC-MS-grade methanol, 2-propanol, acetonitrile, high-performance liquid chromatography-grade chloroform, and 1 M ammonium formate solution were obtained from Wako (Osaka, Japan). Three reagents were used as internal standards (ISs): N-lauroyl-d-erythro-sphingosylphosphorylcholine was from Avanti Polar Lipids (Alabaster, AL, USA), etodolac was from Wako, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine was from Tokyo Chemical Industry (Tokyo, Japan). Water was purified on a Milli-Q system (Merck Millipore, Bedford, MA, USA).
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2

Lipid Extraction and Characterization Protocol

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Ultrapure water, proteomics‐grade hydrochloric acid (HCl), high‐performance liquid chromatography–grade chloroform (CHCl3), methanol (MeOH), acetonitrile (ACN), and isopropanol (IPA) were purchased from Fujifilm Wako Pure Chemical Corp. 9‐Aminoacridine (9‐AA) was purchased from Sigma‐Aldrich. Lipid standards, including LPC (17:1), LPC (16:0), PC (16:0/18:1), and PC (24:0/24:0), were purchased from Avanti Polar Lipids, Inc.
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