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Clb gran 12

Manufactured by Beckman Coulter
Sourced in Germany

The CLB-Gran/12 is a laboratory equipment designed for the analysis of granulocytes, a type of white blood cell. It is capable of performing automated cell counting and differentiation within a blood sample. The core function of this instrument is to provide accurate and reliable data on the granulocyte population, which is crucial for various clinical and research applications.

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2 protocols using clb gran 12

1

Platelet activation and degranulation

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Platelets (10×106/mL) were incubated with TRAP (40 mM) for 30 min at 37°C, and fixed with 4% PFA for 20 min at RT. The expression of CD62P (CLB-Thromb/6, Beckman Coulter) and CD63 (CLB-Gran/12, Beckman Coulter) was determined by flow cytometry (FC) as well as the conformational changes of glycoproteins (GPs) IIb/IIIa complex by PAC-1-antibody (BD Bioscience).
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2

Platelet Activation and Functional Assays

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In order to analyze platelet activation, CSP were stimulated with thrombin receptor-activating peptide-6 (TRAP-6, 10 µM, HART Biologicals, Hartlepool, UK) and incubated with CD62-P (CLB-Thromb/6, Beckman Coulter, Krefeld, Germany) or CD63 (CLB-Gran/12, Beckman Coulter) and measured by flow cytometry (FC) (Navios, Beckman-Coulter, Krefeld, Germany). Light transmission platelet aggregation assay was performed using a four-channel aggregometer (LABiTec, LAbor BioMedical Technologies, Ahrensburg, Germany) to investigate the aggregation ability in response to 20 µM TRAP-6, 1.0 mg/mL ristocetin (HART Biologicals, Hartlepool, UK) or NaCl (Braun, Melsungen, Germany). Next, the adhesion ability of CSP was assessed as previously described using coverslips (Corning, New York, USA) coated with 100 mg/mL fibrinogen (Sigma Aldrich, Munich, Germany) or 5% human serum albumin (Grifols, Berlin, Germany). 9 (link) CSP were allowed to seed on coverslips in the presence of 10 µM TRAP-6. Platelet clot retraction was assessed upon incubation with 7.4 µM CaCl 2 (Sigma Aldrich, Darmstadt, Germany) and thrombin 10 U/mL (Roche, Mannheim, Germany). Thromboelastography was performed using the ClotPro analyzer (Haemonetics, Munich, Germany) according to the manufacturer's instructions. See the Online Supplementary Appendix for further information concerning each assay.
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