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Ms2 sgrna zeo

Manufactured by Addgene
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The MS2-sgRNA-Zeo is a vector that contains the MS2 RNA-binding protein and a zeocin resistance cassette. It is designed for use in CRISPR-Cas9 systems to enable RNA-guided gene regulation.

Automatically generated - may contain errors

Lab products found in correlation

Dcas vp64 blast, by Addgene (2 mentions) Ms2 p65 hsf1 hygro, by Addgene (2 mentions)

Spelling variants of this product

Lenti sgRNA(MS2)_zeo backbone (8 citations) Lenti sgRNA(MS2)_zeo (5 citations) Lenti-sgRNA(MS2)-zeo backbone plasmid (3 citations) Lenti sgRNA(MS2)_zeo plasmid (2 citations)

2 protocols using ms2 sgrna zeo

1

CRISPR-Mediated Activation of MERVL Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
CRISPR activation of MERVL expression was achieved by the Synergistic Activation Mediator (SAM)23 (link) using the three-vector system: dCAS-VP64-Blast (Addgene #61425), MS2-P65-HSF1-Hygro (Addgene #61426) and MS2-sgRNA-Zeo (Addgene #61427), where the non-targeting or MERVL targeted (+317F4 from gag ATG) sgRNAs were cloned using BsmB1 restriction sites. Lentiviruses containing each of the plasmids were generated and ESCs were infected with a 1:1:1 mix of the viruses in presence of 8 μg/ml Polybrene. Infected cells were selected for 48 h with 10 μg/ml of Blasticidin, 250 μg/ml of Hygromycin, and 250 μg/ml Zeocin. Selected cells were collected for total RNA isolation and qPCR analysis.
Guallar D., Bi X., Pardavila J.A., Huang X., Saenz C., Shi X., Zhou H., Faiola F., Ding J., Haruehanroengra P., Yang F., Li D., Sanchez-Priego C., Saunders A., Pan F., Valdes V.J., Kelley K., Blanco M.G., Chen L., Wang H., Sheng J., Xu M., Fidalgo M., Shen X, & Wang J. (2018). RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Nature genetics, 50(3), 443-451.
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2

CRISPR-Mediated Activation of MERVL Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
CRISPR activation of MERVL expression was achieved by the Synergistic Activation Mediator (SAM)23 (link) using the three-vector system: dCAS-VP64-Blast (Addgene #61425), MS2-P65-HSF1-Hygro (Addgene #61426) and MS2-sgRNA-Zeo (Addgene #61427), where the non-targeting or MERVL targeted (+317F4 from gag ATG) sgRNAs were cloned using BsmB1 restriction sites. Lentiviruses containing each of the plasmids were generated and ESCs were infected with a 1:1:1 mix of the viruses in presence of 8 μg/ml Polybrene. Infected cells were selected for 48 h with 10 μg/ml of Blasticidin, 250 μg/ml of Hygromycin, and 250 μg/ml Zeocin. Selected cells were collected for total RNA isolation and qPCR analysis.
Guallar D., Bi X., Pardavila J.A., Huang X., Saenz C., Shi X., Zhou H., Faiola F., Ding J., Haruehanroengra P., Yang F., Li D., Sanchez-Priego C., Saunders A., Pan F., Valdes V.J., Kelley K., Blanco M.G., Chen L., Wang H., Sheng J., Xu M., Fidalgo M., Shen X, & Wang J. (2018). RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Nature genetics, 50(3), 443-451.
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