Normal bsa

Manufactured by Merck Group

Normal BSA is a standard laboratory reagent used for protein quantification. It is a lyophilized powder form of bovine serum albumin (BSA) that provides a consistent reference material for calibrating protein assays.

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Lab products found in correlation

Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Normal goat serum (ngs), by Merck Group (1 mentions) Anti gfap, by Neuromics (1 mentions) Anti neun, by Abcam (1 mentions)

2 protocols using normal bsa

Immunofluorescent Staining of Caveolin-1

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Cells were fixed with 4% paraformaldehyde (Sigma) and maintained in 1% normal BSA (Sigma) to block nonspecific protein binding sites. Cells were incubated with rabbit anticaveolin-1 polyclonal antibody (BD Transduction Laboratories); after a wash, they were incubated with Alexa Fluor 488 goat anti-rabbit IgG (ThermoFisher Scientific) at 1:500 dilution. Stained cells were photographed through a confocal fluorescence microscope (Leica), and images were imported into Adobe Photoshop as TIFFs for figure assembly.

Yamamoto K., Imamura H, & Ando J. (2018). Shear stress augments mitochondrial ATP generation that triggers ATP release and Ca2+ signaling in vascular endothelial cells. American Journal of Physiology - Heart and Circulatory Physiology, 315(5), H1477-H1485.

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Assessing Nanoconjugate Uptake in Brain

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In Figures S11 and S12, we counterstained brain tissue from drug-injected mice to determine if our nanoconjugates are taken up by neurons or glia. Cryosections (8–14 μm thickness) were air-dried for 10 min, fixed with 2% paraformaldehyde for 5 min, and then rinsed three times with PBS. The sections were then incubated in a humid chamber with blocking buffer containing 5% normal BSA, 0.25% Triton X-100, 2% DMSO, and 1% normal goat serum (all from Sigma) in PBS (PBS-T) for 1–2 h at room temperature. The antibodies anti-Neun (Abcam, Cambridge, MA, USA; AB104225) and anti-GFAP (Neuromics, Edina, MN, USA; CH22102) were then diluted 1:500 in PBS-T, and tissue sections incubated simultaneously with antibody solutions overnight at 4 °C in a humid chamber. Tissue sections were then washed five times with PBS-T, incubated in appropriate secondary antibody solutions, and diluted 1:250 in PBS-T, for 2–4 h. After five washes in PBS at room temperature, sections were mounted between coverslips in Fluoromount-G with DAPI.

Israel L.L., Braubach O., Galstyan A., Chiechi A., Shatalova E.S., Grodzinski Z., Ding H., Black K.L., Ljubimova J.Y, & Holler E. (2019). A Combination of Tri-Leucine and Angiopep-2 Drives a Polyanionic Polymalic Acid Nanodrug Platform Across the Blood–Brain Barrier. ACS nano, 13(2), 1253-1271.

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