Cgmp enzyme immunoassay eia kit

Ginsenoside Ro (G-Ro, Figure 1) was obtained from Ambo Institute (Daejeon, Korea). Thrombin was purchased from Chrono-Log Corporation (Havertown, PA, USA). ATP assay kit was purchased from Biomedical Research Service Center (Buffalo, NY, USA). Serotonin ELISA kit was purchased from Labor Diagnostika Nord GmbH & Corporation (Nordhorn, Germany). A-kinase inhibitor Rp-8-Br-cAMPS, G-kinase inhibitor Rp-8-Br-cGMPS, and 2-acetoxymethyl (Fura 2-AM) were obtained from Sigma Chemical Corporation (St. Louis, MO, USA). Lactate dehydrogenase (LDH) cytotoxicity assay kit, cAMP enzyme immunoassay (EIA) kit, and cGMP enzyme immunoassay (EIA) kit were obtained from Cayman Chemical (Ann Arbor, MI, USA). Anti-IP₃-receptor type I, antiphosphor-IP₃-receptor type I (Ser¹⁷⁵⁶), anti-ERK (1/2), antiphosphor-ERK (1/2), anti-rabbit IgG-horseradish peroxidase conjugate (HRP), and lysis buffer were obtained from Cell Signaling (Beverly, MA, USA). Anti-β-actin was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal to CD62P (p-selectin) antibody was purchased from Biolegend (San Diego, CA, USA). Polyvinylidene difluoride (PVDF) membrane was from GE Healthcare (Piscataway, New Jersey, USA). Enhanced chemiluminescence solution (ECL) was from GE Healthcare (Chalfont St. Giles, Buckinghamshire, UK).

cGMP concentration in the pulmonary arterial tissues was measured by a commercially available cGMP enzyme immunoassay (EIA) kit (Cayman). The excised pulmonary arteries were pulverized and homogenized with 5% trichloroacetic acid (TCA). The samples were centrifuged at 1,500 ×g for 30 min at 4°C and the supernatant was collected. The supernatants were analyzed according to the manufacturer’s instructions.