Antibodies against nfatc1

Berberine sulfate (Purity > 98%) was purchased from Mansite (Chengdu, China), and dissolved in Dimethyl sulfoxide (DMSO). α Modification of Minimal Essential Medium (α-MEM) and fetal bovine serum (FBS) was purchased from TRACE (Sydney, Australia). Recombinant GST-rRANKL protein was expressed and purified as previously described [26 (link)] and recombinant macrophage colony stimulating factor (M-CSF) was obtained from R&D Systems (Minnneapolis, MN, USA). MTS reagent and luciferase analysis reagents were obtained from Promega (Sydney, Australia). Antibodies against NFATc1, IκBα, ERK, phosphorylated ERK, and β-actin were obtained from Santa Cruz Biotechnology (Dallas, CA, USA). Tartrate resistant acid phosphatase (TRAcP) enzymatic activity was detected using the Leukocyte acid phosphatase staining kit (Sigma, St. Louis, MO, USA).

BCI hydrochloride (also called BCI or (E)-BCI)) was obtained from MCE (Shanghai, China) and dissolved in DMSO. DMEM, α-MEM, and FBS were purchased from Hyclone (UT, United States). Recombinant murine RANKL and M-CSF were purchased from R&D (MN, United States). Primary antibodies specific for β-tubulin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), phospho-NF-kB/p65, NF-kB/p65, p-IkBα, IkBα, p38, p-ERK, ERK, CTSK, MMP9, and c-Fos were obtained from Proteintech (Wuhan, China). Primary antibodies specific for p-JNK, JNK, and p-p38 were obtained from Affinity Biosciences (Zhenjiang, China). Primary antibodies specific for p-STAT3, and STAT3 were from Bioworld (Nanjing, China). Antibodies against NFATc1 were obtained from Santa Cruz (TX, United States). Anti-rabbit and anti-mouse HRP-labeled secondary antibodies were obtained from Beyotime (Shanghai, China).