Hrp conjugated anti rabbit or anti mouse abs

Manufactured by Jackson ImmunoResearch

Sourced in United Kingdom

HRP-conjugated anti-rabbit or anti-mouse Abs are secondary antibodies that are conjugated with horseradish peroxidase (HRP). They are designed to detect and bind to primary antibodies raised in rabbit or mouse, respectively. The HRP enzyme can then be used to catalyze a colorimetric or chemiluminescent reaction, allowing for the visualization and detection of the target antigen.

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Lab products found in correlation

Anti pfak, by Thermo Fisher Scientific (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Immune blot pvdf membrane, by Bio-Rad (1 mentions) Ecl chemiluminescence method, by Thermo Fisher Scientific (1 mentions) Anti tubulin, by GeneTex (1 mentions) Anti igm f ab 2 fragment, by Jackson ImmunoResearch (1 mentions) Protease inhibitor mixture, by Roche (1 mentions) Mini protean system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Rabbit anti-HRP (12 citations) HRP anti-mouse (5 citations)

2 protocols using hrp conjugated anti rabbit or anti mouse abs

Western Blot Analysis of Signaling Proteins

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Educated BMMCs were collected by scratching in 10 mL of PBS. After centrifugation, the proteins were isolated using RIPA buffer supplemented with protease and phosphatase inhibitors. 20μL of the obtained lysate were separated by NUPAGE 4-12% Bis-Tris Gel electrophoresis (SDS- PAGE) (Gibco; Amarillo/TX/USA) and transferred to a Immune-Blot PVDF membrane (Biorad; Hercules/Californie/USAs). After transfer, the immune-blots were blocked by incubating with 5% BSA in Tris buffered saline (TBS) (Euromedex; Souffelweyersheim/France) containing 0.05% Tween 20 (VWR; Radnor/Pennsylvanie/USA). The blots were then probed overnight with appropriately diluted primary Abs. After washing in TBS-Tween 0.05%, the membranes were revealed with secondary antibodies for 1 hour at room temperature. After washing, the blots were developed using the ECL chemiluminescence method according to the manufacturer’s protocol (Pierce Chemical; Waltham/MA/USA).
The following antibodies were used to detect their corresponding substrates: anti-pFAK from Invitrogen, FAK from Cell Signalling, pERK from Cell Signaling and anti-tubulin obtained from GeneTex. Either HRP-conjugated anti-rabbit or anti-mouse Abs (Jackson Immunoresearch; Ely/United Kingdom) were used for primary antibody binding.

Bachy S., Wu Z., Gamradt P., Thierry K., Milani P., Chlasta J, & Hennino A. (2022). βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer. iScience, 25(2), 103758.

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B Cell Signaling Pathway Analysis

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Purified B cells were left at 37°C for 15 min in chamber buffer (PBS, 0.5% FCS, 1 g l⁻¹ D-glucose, 2 mM MgCl₂, 0.5 mM CaCl₂) to equilibrate prior to stimulation. They were then stimulated for various times with 10 μg ml anti-IgM F(ab′)2 fragment (Jackson ImmunoResearch). Stimulated cells were lysed in lysis buffer [20 mM Tris-HCl (pH 8), 150 mM NaCl, 5 mM EDTA, Protease Inhibitor mixture (Roche), 10 mM NaF, 1 mM Na₃VO₄, 1% NP40] for 30 min on ice and samples loaded into Tris Glycin gels prior to electrophoresis using the miniprotean system (Bio-Rad). Proteins were detected with Abs against pErk, pAkt (S473), pCD19, pSyk, pSrc, total Erk (all from Cell Signaling Technology), tubulin (Sigma-Aldrich), or TC10 (Proteintech) using the secondary HRP-conjugated anti-rabbit or anti-mouse Abs (Jackson ImmunoResearch). Blot densitometry analysis was performed using Image J software.

Burbage M., Keppler S.J., Montaner B., Mattila P.K, & Batista F.D. (2017). The Small Rho GTPase TC10 Modulates B Cell Immune Responses. The Journal of Immunology Author Choice, 199(5), 1682-1695.

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