Sodium chloride (nacl)

Manufactured by Fagron

Sourced in Belgium

NaCl is a laboratory equipment item that serves as a source of sodium (Na) and chloride (Cl) ions. It is a common salt used in various scientific applications and experiments.

Automatically generated - may contain errors

Lab products found in correlation

Calcium chloride dihydrate, by Chempur (1 mentions) Sodium chloride (nacl), by Chempur (1 mentions) Potassium dihydrogen phosphate, by Chempur (1 mentions) Glycerol, by Fagron (1 mentions) Glycine, by Merck Group (1 mentions) L histidine, by Merck Group (1 mentions) Trehalose, by Cargill (1 mentions) Mannitol, by Fagron (1 mentions) Lactose, by Fagron (1 mentions) Nah2po4 2h2o, by Merck Group (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Na2hpo4 2h2o, by Merck Group (1 mentions) Freestyle 293 expression medium, by Thermo Fisher Scientific (1 mentions) Tris hcl, by Thermo Fisher Scientific (1 mentions) Hepes, by Serva Electrophoresis (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions) Percoll, by GE Healthcare (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)

5 protocols using sodium chloride (nacl)

Chitosan and Pectin Hydrogel Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Medium-molecular-weight CS 80/50 (degree of deacetylation: 77.6–82.5%, viscosity of 1% solution in 1% acetic acid: 31–70 mPa·s, molecular weight: 80–200 kDa) was obtained from Heppe Medical CS GmbH (Haale, Germany). Low-methoxy amidated PC with low- (a type CF 010, degree of esterification 34%, degree of amidation 17%, pH 4.2 for 2.5% solution in distilled water at 20 °C) and high-calcium reactivity (a type CF 020, degree of esterification 31%, degree of amidation 19%, pH 4.1 for 2.5% solution in distilled water at 20 °C) were kindly gifted by Herbstreith & Fox and GmbH & Co. KG (Neuenbürg, Germany). Potassium dihydrogen phosphate, sodium chloride, calcium chloride dihydrate, and 85% lactic acid were purchased from Chempur (Piekary Śląskie, Poland). Glycerol was obtained from Fagron (Kraków, Poland).
SSS composed of Potassium dihydrogen phosphate (1.63 mg/mL), sodium chloride (2.32 mg/mL), and calcium chloride dihydrate (0.22 mg/mL) with pH 6.2 adjusted by disodium hydrogen phosphate addition (according to Nair et al. with modification [25 (link)]) was used for the research analyses.

Potaś J., Wilczewska A.Z., Misiak P., Basa A, & Winnicka K. (2022). Optimization of Multilayer Films Composed of Chitosan and Low-Methoxy Amidated Pectin as Multifunctional Biomaterials for Drug Delivery. International Journal of Molecular Sciences, 23(15), 8092.

+ Open protocol

+ Expand

Freeze-Drying of Protein Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five different formulations having a different specific dry product resistance were selected from literature (Kuu et al., 2006; Overcashier et al., 1999) (Table 1). Trehalose was purchased from Cargill (Germany). Polysorbate 20, sodium chloride, lactose and mannitol were purchased from Fagron (Belgium). L-histidine and glycine were purchased from Sigma-Aldrich (United States).
Prior to freeze-drying, 10ml type I glass vials were filled with a specific volume of the formulation (see section 4.2).
After Freezing (see section 4.1.), all frozen vials were dried in an Amsco FINN-AQUA GT4 freeze-dryer (GEA, Köln, Germany).

De Meyer L., Van Bockstal P.J., Corver J., Vervaet C., Remon J.P, & De Beer T. (2015). Evaluation of spin freezing versus conventional freezing as part of a continuous pharmaceutical freeze-drying concept for unit doses. International journal of pharmaceutics, 496(1).

+ Open protocol

+ Expand

Caco-2 Cell Adhesion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The growth conditions of the Caco‐2 cultures and adhesion assays were carried out as described previously (Lebeer et al., 2012). Caco‐2 cells were incubated with 1 ml fresh bacterial culture or rehydrated spray‐dried powder [10⁷ CFU ml⁻¹ in Dulbecco's Modified Eagle Medium (Life Technologies, Waltham, MA, USA) without 10% foetal bovine serum (Thermo Fisher, Asse, Belgium)] for 1 h at 37°C, 5% CO₂, 100% humidity. Then, the cells were washed by adding prewarmed phosphate‐buffered saline (PBS), containing 8.2 g l⁻¹ NaCl (Fagron, Waregem, Belgium), 0.3 g l⁻¹ NaH₂PO₄.2H₂O (Merck kGaA, Darmstadt, Germany) and 1.54 g l⁻¹ Na₂HPO₄.2H₂O (Merck kGaA). To determine the number of viable adhered bacteria, appropriated serial dilutions of the remained adhered bacterial cells in PBS were plated n MRS agar in triplicate and the CFU were enumerated after 48 h. The adhesion ratio was calculated by comparing the number of cells present in the bacterial suspension which was added initially to the Caco‐2 cells, to the bacterial colonies counted that adhered to the Caco‐2 cells.

Kiekens S., Vandenheuvel D., Broeckx G., Claes I., Allonsius C., De Boeck I., Thys S., Timmermans J., Kiekens F, & Lebeer S. (2019). Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG. Microbial Biotechnology, 12(5), 849-855.

+ Open protocol

+ Expand

Protein Expression in FreeStyle 293

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tris-HCl was from Invitrogen (Breda, the Netherlands), NaCl was obtained from Fagron (Rotterdam, the Netherlands) and HEPES was from Serva (Heidelberg, Germany), FreeStyle 293 expression medium was obtained from Gibco (Thermo Fisher Scientific). Tween-20 and D₂O was from Sigma-Aldrich (St Louis, MO, USA). Human serum albumin (HSA) was obatined from the Division of Products at Sanquin (Amsterdam, the Netherlands). All other chemicals were from Merck (Darmstadt, Germany), unless indicated otherwise.

Przeradzka M.A., van Galen J., Ebberink E.H., Hoogendijk A.J., van der Zwaan C., Mertens K., van den Biggelaar M, & Meijer A.B. (2020). D’ domain region Arg782-Cys799 of von Willebrand factor contributes to factor VIII binding. Haematologica, 105(6), 1695-1703.

+ Open protocol

+ Expand

Neutrophil Isolation from Human Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heparinized venous blood was drawn from healthy individuals, and neutrophils were isolated, as previously described (12 (link)). All human blood samples were obtained after informed consent, and all experiments involving human blood samples were conducted in accordance with the 2013 Declaration of Helsinki. Blood was first diluted approximately 1:1 in phosphate-buffered saline (PBS) + 10% trisodium citrate (TNC) and loaded on isotonic Percoll for gradient centrifugation (1.076 g/mL, GE Healthcare). After centrifugation (20 min, 938 g, room temperature), the pellet fraction was lysed using an ice-cold hypotonic ammonium chloride solution (155 mM NH₄Cl (Merck), 10 mM KHCO₃ (Merck), and 0.1 mM EDTA (Merck) in H₂O (Gibco)) to lyse erythrocytes. Neutrophils were then thoroughly washed with PBS and reconstituted to a concentration of 5 × 10⁶ cells/mL in HEPES+ medium (20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Sigma Aldrich), 132 mM NaCl (Fagron), 6 mM KCl (Merck), 1 mM MgSO₄ (Merck), 1.2 mM K₂HPO₄ (Merck), 7 H₂0 (Gibco), pH 7.4 with 10 M NaOH), supplemented with 5 g/L human albumin (Albuman, Sanquin Plasma Products), and 5.5 mM glucose (Merck), and 1 M calcium (Calbiotech) (43 ).

Bouti P., Klein B.J., Verkuijlen P.J., Schornagel K., van Alphen F.P., Taris K.K., van den Biggelaar M., Hoogendijk A.J., van Bruggen R., Kuijpers T.W, & Matlung H.L. (2024). SKAP2 acts downstream of CD11b/CD18 and regulates neutrophil effector function. Frontiers in Immunology, 15, 1344761.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!