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2 protocols using anti mouse tcrβ

1

Multicolor Flow Cytometry Antibodies

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Fluorophore-conjugated monoclonal anti-mouse CD3ε (FITC, 145-2C11), anti-mouse Gr1 (APC, 1A8), anti-mouse B220 (PerCP-Cy5.5, RA3-6B2), anti-mouse CD11b (PE, M1/70), anti-mouse CD45 (APC-Cy7, 30-F11), anti-mouse TCRβ (FITC, H57-597), anti-mouse TCRγδ (APC, GL3), anti-mouse CD4 (PerCP-Cy5.5, RM4-5), anti-mouse IL17A (PE, 17B7), anti-mouse CD11b (FITC, M1/70), anti-mouse CD11c (PerCP-Cy5.5, N418), anti-mouse CD49b (APC, DX5) were purchased from eBioscience (San Diego, CA, USA), Bio Legend (San Diego, CA, USA) or TONBO Bioscience (San Diego, CA, USA).
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2

Isolation and Characterization of Mouse Immune Cells

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Antibodies including anti-mouse TCRβ, CD4, CD8, CD44, CD62L, H-2Kb, H-2Kd, CD122, CD69, CD137, MHC-II, CD86, CD70, CD24, CD172a, and B220 were purchased from eBioscience. CD90.2 microbeads and negative mouse CD8+ T cells isolation kits were purchased from Miltenyi Biotec and Stem Cell Company respectively. The mouse CD11c+ cell isolation kits for CD11c+ negative selection were purchased from Stem Cell Company.
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