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Cd19 alexa fluor 700

Manufactured by Thermo Fisher Scientific
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CD19-Alexa Fluor 700 is a fluorescently labeled antibody that binds to the CD19 surface marker. It is a tool for the detection and analysis of CD19-positive cells in flow cytometry applications.

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Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions) Aria 3, by BD (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 apc cy7, by BD (1 mentions) Cd19 apc cy7, by BD (1 mentions) F4 80 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd11b percp cy5, by BD (1 mentions) Cxcr5 biotin, by BD (1 mentions) Cd8 pacific orange, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by Thermo Fisher Scientific (1 mentions) Nk1.1 pe cy7, by Thermo Fisher Scientific (1 mentions) Ly6g percp cy5, by BD (1 mentions) Ox40 apc, by Thermo Fisher Scientific (1 mentions) Cd8 pacific blue, by BD (1 mentions) Cd27 apc, by BD (1 mentions) Cd3 alexa fluor 700, by BD (1 mentions) Hla dr fitc, by BD (1 mentions) Cd38 pe, by BD (1 mentions) Cd4 pe cy7, by BD (1 mentions) Cd25 apc, by BD (1 mentions)

Spelling variants of this product

Alexa Fluor 700 (33 citations) Alexa Fluors (21 citations)

2 protocols using cd19 alexa fluor 700

1

Multiparameter Flow Cytometry Profiling

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Lymphocytes and myeloid cells were prepared as above. Cells were stained according to standard protocols with combinations of the following anti-mouse antibodies: OX40-APC (eBioscience; clone, OX86), CD4-FITC (eBioscience; clone, GK1.5), CD4-APC-Cy7 (BD Biosciences; clone, GK1.5), CD8–Pacific Orange (Life Technologies; clone, 5H10), CD8–Pacific Blue (BD Biosciences; clone, 53-6.7), TCRb–Pacific Blue (BD Biosciences; clone, H57-597), TCRb-PE (BD Biosciences; clone, H57-597), NK1.1-PE-Cy7 (eBioscience; clone, PK136), CD19–Alexa Fluor 700 (eBioscience; clone, ebio1D3), CD19-APCCy7 (BD Biosciences; clone, 1D3), CXCR5-biotin (BD Biosciences; clone, 2G8), OX40L-PE (eBioscience; clone, RM134L), F4/80-PE-Cy7 (eBioscience; clone, BM8), CD11b-PerCP-Cy5.5 (BD Biosciences; clone, M1/70), CD11c-APC (eBiosciences; clone, N418), major histocompatibility complex II–efluor 450(I-A/I-E) (eBioscience; clone, M5/114.15.2), Ly6g-PerCP-Cy5.5 (BD Biosciences; clone, 1A8), and Ly6c-AF700 (BD Biosciences, clone: AL-21). When applicable for biotinylated antibodies, cells were stained with secondary QDot 605–conjugated streptavidin (Life Technologies). Cells were analyzed using an LSR II flow cytometer (BD Biosciences) and FlowJo software (TreeStar) or sorted on an Aria III (BD Biosciences).
Publicover J., Gaggar A., Jespersen J.M., Halac U., Johnson A.J., Goodsell A., Avanesyan L., Nishimura S.L., Holdorf M., Mansfield K.G., Judge J.B., Koshti A., Croft M., Wakil A.E., Rosenthal P., Pai E., Cooper S, & Baron J.L. (2018). An OX40/OX40L interaction directs successful immunity to hepatitis B virus. Science translational medicine, 10(433), eaah5766.
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2

Multiparametric Flow Cytometry Analysis of PBMCs

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PBMCs were resuspended in PBS, containing 0.5% w/v BSA and 0.01% sodium azide. PBMCs were incubated with saturating concentrations of fluorescently labeled conjugated mAbs. Analysis of cells was performed using a FACSCanto‐II flowcytometer and FlowJo software (version 9.1 and 10) and for the methods of flow cytometry we adhered to the ‘Guidelines for the use of flow cytometry and cell sorting in immunological studies’ 38. The following mAbs were used for flow cytometry: CD3 Alexa Fluor 700 [557943], CD4 PE‐Cy7 [348809], CD8 PerCP‐Cy5.5 [341050], CD19 Alexa Fluor 700 [557921], CD19 PerCP‐Cy 5.5 [332780], CD20 APC‐H7 [641414], CD20 PerCP‐Cy5.5 [332781], CD25 APC [340907], CD27 APC [337169], CD38 PE [345806], CD38 PE‐Cy7 [335825], HLA‐DR FITC [347400], and IgD PE [555779] from BD (San Jose, USA); CD3 Alexa 700 [56‐0038‐41], CD19 Alexa Fluor 700 [56‐0199‐42], and CD27 APC‐eFluor 780 [47‐0279‐42] from eBioscience (San Diego, USA); and CD27 FITC [M1764] from Sanquin (Amsterdam, the Netherlands). To assess lymphocyte viability TO‐PRO‐3 iodide [T3605] was used (Thermo Fisher Scientific, Massachusetts, USA).
Tuijnenburg P., aan de Kerk D.J., Jansen M.H., Morris B., Lieftink C., Beijersbergen R.L., van Leeuwen E.M, & Kuijpers T.W. (2019). High‐throughput compound screen reveals mTOR inhibitors as potential therapeutics to reduce (auto)antibody production by human plasma cells. European Journal of Immunology, 50(1), 73-85.
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