Analysis of cell populations in BALF or single cell suspension of lung homogenate was conducted as before46 (link). Cells were labeled with a combination of phycoerythrin (PE)-labeled anti-NK 1.1 (PK136; eBioscience), fluorescein (FITC)-labeled anti-Ly6G (RB6-8C5; eBioscience), allophycocyanin (APC)-labeled anti-MHCII (M5/114.15.2; eBioscience), PerCP-Cy5.5-labelled anti-CD11c (N418; Biolegend), or phycoerythrin (PE)-labeled CD4 (RM4-5; Biolegend) alone. Neutrophils (PMNs) were defined as Ly6G+/MHCII− , Macrophages as CD11c+/MHCIIlow-mid and Dendritic cells (DCs) as CD11c+/MHCIIhigh. Fc block (anti-mouse CD16/32) was also added to each sample (93; eBioscience). Uniform dyed microspheres (Bangs Laboratories, Inc) were added to calculate the concentration of cellular components. Samples were run on FACS Calibur and analyzed on FlowJo (ver 10.0.6).
Fitc labeled anti ly6g rb6 8c5
Manufactured by Thermo Fisher Scientific
The FITC)-labeled anti-Ly6G (RB6-8C5) is a flow cytometry reagent used for the identification and enumeration of mouse Ly6G-positive cells. It is a fluorescently-conjugated monoclonal antibody that binds to the Ly6G antigen expressed on the surface of certain immune cells.
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Lab products found in correlation
2 protocols using fitc labeled anti ly6g rb6 8c5
1
Analysis of Lung Immune Cells by Flow Cytometry
2
Analysis of Lung Immune Cells by Flow Cytometry
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Analysis of cell populations in BALF or single cell suspension of lung homogenate was conducted as before46 (link). Cells were labeled with a combination of phycoerythrin (PE)-labeled anti-NK 1.1 (PK136; eBioscience), fluorescein (FITC)-labeled anti-Ly6G (RB6-8C5; eBioscience), allophycocyanin (APC)-labeled anti-MHCII (M5/114.15.2; eBioscience), PerCP-Cy5.5-labelled anti-CD11c (N418; Biolegend), or phycoerythrin (PE)-labeled CD4 (RM4-5; Biolegend) alone. Neutrophils (PMNs) were defined as Ly6G+/MHCII− , Macrophages as CD11c+/MHCIIlow-mid and Dendritic cells (DCs) as CD11c+/MHCIIhigh. Fc block (anti-mouse CD16/32) was also added to each sample (93; eBioscience). Uniform dyed microspheres (Bangs Laboratories, Inc) were added to calculate the concentration of cellular components. Samples were run on FACS Calibur and analyzed on FlowJo (ver 10.0.6).
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