1260 infinity 2 multicolumn thermostat

The chromatographic analyses were performed using an Agilent 1260 Infinity II HPLC (Agilent, Santa Clara, CA, USA) equipped with a 1260 Infinity II Quaternary Pump (model G7111A), 1260 Infinity II auto-sampler (model G7129A), 1260 Infinity II Multicolumn Thermostat (model G7116A), and 1260 Infinity II Diode Array Detector (model G7115A). The results were collected and integrated using Agilent OpenLAB CDS LC ChemStation software. The selected column was a Poroshell 120 EC-C18 (150 × 4.6 mm i.d., particle size 4 μm; Agilent, Santa Clara, CA, USA), working at 20 °C. Samples were dissolved in acetonitrile (ACN) (1 mg/mL) and analyzed using as the mobile phase a mixture of water (channel A) and acetonitrile (channel B), both containing 0.1% v/v of trifluoroacetic acid (TFA). The gradient elution started from 90% of A to reach the 90% of B over 6 min at a flow rate of 0.8 mL/min, for a total run of 15 min. The UV detector was set at a length of 254 nm. HPLC chromatograms of 1d and 1h, reported in the supporting information (Figures S1 and S2), confirmed a high grade of purity (99%).

The liquid chromatography
system was an Agilent 1260 Infinity II HPLC (Agilent, Santa Clara,
CA) consisting of a 1260 Infinity II Quaternary Pump (model G7111A),
1260 Infinity II autosampler (model G7129A), a 1260 Infinity II Multicolumn
Thermostat (model G7116A), and a 1260 Infinity II Diode Array Detector
(model G7115A). Data were acquired and integrated using the software
Agilent OpenLab CDS LC ChemStation. The separation was performed using
a Poroshell 120 EC-C18 (150 × 4.6 mm i.d., particle size 4 μm;
Agilent, Santa Clara), maintained at 20 °C. The samples were
run using a mixture of water (A) and acetonitrile (B) enriched with
trifluoroacetic acid (0.1% v/v). The gradient used was from 80% A
to 100% B over 10 min. The flow rate was 0.8 mL/min. The UV detector
was set at a length of 254 nm. Enantioselective HPLC analyses were
performed using the same above-described conditions except for the
stationary phase, which was a Chiralcel OJ[-RH] column (150 ×
4.6 mm, 5 μm).