SARS-CoV-2 spike-pseudotyped lentiviral particles, VSV-G-pseudotyped particles and human ACE2-expressing HEK293 cells were purchased from GeneCopoeia, Rockville, MD. Recombinant COVID-19 Spike protein S1 was purchased from Abeomics, San Diego, CA. While anti-SARS-CoV-2 Spike S1 antibody was bought from BioVision (Milpitas, CA), anti-human ACE2 antibody was purchased from R&D Systems (Minneapolis, MN). Anti-human MyD88 antibody was bought from Millipore (Burlington, MA). Human A549 lung carcinoma cell line and F-12K medium were obtained from ATCC, Manassas, VA. Hank’s balanced salt solution, 0.05% trypsin, and antibiotic-antimycotic mixture were bought from Mediatech (Washington, DC). Fetal bovine serum (FBS) was obtained from Atlas Biologicals, Fort Collins, CO. ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit was purchased from BPS Bioscience, San Diego, CA. Anti-human IL-6 antibody and human IL-1β and IL-6 ELISA kits were bought from ThermoFisher, Waltham, MA.
Human ace2 expressing hek293 cells
Manufactured by GeneCopoeia
The Human ACE2-expressing HEK293 cells are a cell line derived from human embryonic kidney 293 cells that have been engineered to express the human angiotensin-converting enzyme 2 (ACE2) protein. ACE2 is a receptor that serves as the entry point for the SARS-CoV-2 virus, which causes COVID-19. These cells can be used for research purposes related to the interaction between ACE2 and SARS-CoV-2, as well as for the development and testing of potential therapeutics targeting this interaction.
Automatically generated - may contain errors
Lab products found in correlation
Sars cov 2 spike pseudotyped lentiviral particles, by GeneCopoeia (2 mentions)
Vsv g pseudotyped particles, by GeneCopoeia (2 mentions)
Human a549 lung carcinoma cell line, by American Type Culture Collection (2 mentions)
Ace2 sars cov 2 spike inhibitor screening assay kit, by BPS Biosciences (2 mentions)
F 12k medium, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (2 mentions)
Anti sars cov 2 spike s1 antibody, by Abcam (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Eugenol, by Merck Group (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Recombinant human ace2 protein 18 739, by MyBioSource (1 mentions)
Methylcellulose, by Spectrum Chemical (1 mentions)
2 protocols using human ace2 expressing hek293 cells
1
SARS-CoV-2 Spike Protein Inhibition Assay
2
Screening Inhibitors of SARS-CoV-2 Spike-ACE2 Interaction
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Eugenol, ursolic acid, oleanolic acid, and β-caryophylline were purchased from Sigma, St. Louis, MO. Methyl cellulose was bought from Spectrum Chemical, Gardena, CA. SARS-CoV-2 spike-pseudotyped lentiviral particles, VSV-G-pseudotyped particles and human ACE2-expressing HEK293 cells were purchased from GeneCopoeia, Rockville, MD. Recombinant COVID-19 Spike protein S1 (14-685) was purchased from Abeomics, San Diego, CA. Recombinant human ACE2 protein (18-739) was purchased from MyBiosource, San Diego, CA. Human A549 lung carcinoma cell line and F-12 K medium were obtained from ATCC, Manassas, VA. Hank’s balanced salt solution, 0.05 % trypsin, and antibiotic-antimycotic mixture were bought from Mediatech (Washington, DC). Fetal bovine serum (FBS) was obtained from Atlas Biologicals, Fort Collins, CO. ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit was purchased from BPS Bioscience, San Diego, CA. Mouse TNFα ELISA kit was bought from ThermoFisher, Waltham, MA.
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