Anti il 4 clone 11b11

Manufactured by BD

Sourced in United States

Anti-IL-4 (clone 11B11) is a monoclonal antibody that binds to interleukin-4 (IL-4), a cytokine involved in the regulation of immune responses. This product is intended for research use only.

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Lab products found in correlation

Intracellular staining kit, by BD (1 mentions) Anti foxp3 clone fjk 16s, by Thermo Fisher Scientific (1 mentions) Anti cd80 clone 16 10a1, by BioLegend (1 mentions) Anti ifn γ clone xmg1, by BD (1 mentions) Anti cd11c clone n418, by BioLegend (1 mentions) Facscalibur, by BD (1 mentions) Cytofix cytoperm, by BD (1 mentions) Cellquest pro software, by BD (1 mentions) Ionomycin, by Merck Group (1 mentions) Golgistop, by BD (1 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Anti cd3 clone 145 2c11, by BD (1 mentions) Htgf β, by R&D Systems (1 mentions) Cd25 microbead kit, by Miltenyi Biotec (1 mentions) Anti cd28 clone 37, by BD (1 mentions) Anti ifn γ clone xmg1, by Thermo Fisher Scientific (1 mentions) Facscanto 2 flow cytometry, by BD (1 mentions)

Spelling variants of this product

Anti-IL-4 (53 citations) Anti-IL-4 (11B11) (6 citations) IL-4 (11B11; (5 citations)

4 protocols using anti il 4 clone 11b11

Phenotypic and Functional Analysis of DCs

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For phenotypic analysis, the surface molecules of DCs were stained with monoclonal antibody-conjugated fluorescent agent. Cells were incubated with the following antibodies at 4 °C for 20 min: anti-CD11c (clone N418, allophycocyanin (APC) or phycoerythrin (PE)), anti-CD80 (clone 16-10A1, PE), anti-CD86 (clone PO3, fluorescein-5-isothiocyanate (FITC)), and anti-MHC II (clone 2G9, PE) (BioLegend or eBioscience, San Diego, CA, USA). To examine T cell subpopulations, cells were stained with APC-conjugated anti-CD4 (clone RM4-5), and then cells were fixed and permeabilized using an Intracellular Staining Kit (BD Biosciences, San Diego, CA, USA or Invitrogen, Waltham, MA, USA). For intracellular staining, anti-IFN-γ (clone XMG1.2, PE), anti-IL-4 (clone 11B11, Alexa Fluor 488), or anti-IL-17A (clone TC11-18H10.1, PE) (all from BD Bioscience) plus anti-Foxp3 (clone FJK-16s; Invitrogen, PE) antibodies were used. FlowJo v.10.6.2 software (FlowJo, Ashland, OR, USA) was used to analyze the data.

Oh J.S., Hwang S.U., Noh K.E., Lee J.H., Choi S.Y., Nam J.H., Song M.S., Jung N.C., Song J.Y., Seo H.G., Na Y, & Lim D.S. (2022). Synthetic TGF-β Signaling Agonist-Treated Dendritic Cells Induce Tolerogenicity and Antirheumatic Effects. Current Issues in Molecular Biology, 44(9), 3809-3821.

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CGRP Modulates T Cell Responses

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pDMECs were treated with 100 nM CGRP or medium alone for 3 h, washed 4 times, and then co-cultured with LCs and CD4⁺ T cells (from DO11.10 Tg mice) in the presence of 10 μM OVA_323–339 for 48 h. For the last 5 h of co-culture, cells were stimulated with 50 ng/ml phorbol myristate acetate and 750 ng/ml ionomycin (Sigma-Aldrich). After 1 h, GolgiStop (BD Biosciences) was added to block cytokine secretion. LCs still bound to beads were then removed by magnetic capture. CD4⁺ T cells were surface stained for 20–30 minutes at 4°C with PerCP-Cy 5.5-labled anti-CD4 mAb (BD Biosciences) in PBS supplemented with 0.1% bovine serum albumin (BSA) and 0.1% sodium azide. After fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences), cells were stained with Alexa Fluor 647-labeled anti-IL-17 and fluorescein isothiocyanate (FITC) labled anti-IFN-γ (clone XMG1.2; BD Biosciences), phycoerythrin (PE) or Alexa Fluor 647-lableled anti-IL-17A (clone TC11–18H10; BD Biosciences), anti-IL-4 (clone 11B11, BD Biosciences) monoclonal antibodies. Analysis was performed on a FACSCalibur (BD Biosciences). Data analysis was conducted using CellQuest Pro software (BD Biosciences).

Ding W., Stohl L.L., Xu L., Zhou X.K., Manni M., Wagner J.A, & Granstein R.D. (2016). Calcitonin Gene-Related Peptide-Exposed Endothelial Cells Bias Ag Presentation to CD4+ T Cells Toward a Th17 Response. Journal of immunology (Baltimore, Md. : 1950), 196(5), 2181-2194.

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Regulatory T Cell Differentiation Assay

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CD4⁺ T cells were purified from naïve mice using the CD4⁺ T cell isolation kit (Miltenyi Biotech, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. Regulatory T cells were depleted using the CD25 MicroBead kit (Miltenyi Biotech, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. Purified CD4⁺ CD25^- T cells were cultured in IMDM supplemented with 5% fetal bovine serum, glutamax, sodium pyruvate, non-essential amino acids and antibiotics in 48-well plates at a density of 0.5 × 10⁶ cells per well. Wells were pre-coated with 5 μg/ml anti-CD3 (clone 145-2C11, BD Biosciences, San Diego, CA, USA) at 37°C for 4 hours and rinsed twice with PBS before applying cells. To induce regulatory T cell differentiation, cells were stimulated with 1 μg/ml anti-CD28 (clone 37.51, BD Biosciences, San Diego, CA), 10 ng/ml hTGFβ (R&D Systems, Minneapolis, MN, USA), 10 μg/ml anti-IFNγ (clone XMG1.2, eBioscience, San Diego, CA, USA) and 10 μg/ml anti-IL-4 (clone 11B11, BD Biosciences, San Diego, CA, USA). Mepazine was omitted or added at a concentration of 5 μM or 10 μM. Cells were analyzed for Foxp3 expression at day 3 and day 5 in culture.

Mc Guire C., Elton L., Wieghofer P., Staal J., Voet S., Demeyer A., Nagel D., Krappmann D., Prinz M., Beyaert R, & van Loo G. (2014). Pharmacological inhibition of MALT1 protease activity protects mice in a mouse model of multiple sclerosis. Journal of Neuroinflammation, 11, 124.

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Murine RSV M2 Peptide-Specific CD8+ T-cell Phenotyping

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The murine cells were incubated with a specific tetramer to the peptide of the M2 protein (SYIGSINNI) from RSV (M282-90) and were stained with the following surface antibodies: anti-CD8+ (Percp and PE-Cy7) (clone 53,67), anti-CD127 (PE-Cy7) (clone SB/199), anti-KLRG1 (APC) (clone 2FI), anti-CD103 (PE) (clone: M290), anti-CD44 (PerCp) (clone: IM7), anti-CD62L (APC) (clone: MEL-14) (BD Biosciences®), anti-IL-10 (APC) (clone JES5-16E), anti-IL4 (clone 11B11), and anti-IFNγ (ALEXA 647) (clone XMG1.2) (BD Biosciences®). Samples were acquired by FACS CANTO II flow cytometry (BD Bioscience®). The FlowJo software (TreeStar®) was used for data analysis.

de Freitas D.D.N., Marinho Franceschina C., Muller D., Hilario G.T., Gassen R.B., Fazolo T., de Lima Kaminski V., Bogo Chies J.A., Maito F., Antunes K.H., Zanin R.F., Rodrigues LC J.r, & Duarte de Souza A.P. (2021). RvD1 treatment during primary infection modulates memory response increasing viral load during respiratory viral reinfection. Immunobiology, 226(6).

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