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2 protocols using cariprazine

1

Neurochemical Screening for Drug Evaluation

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Aripiprazole, Clozapine, Quetiapine, Olanzapine, Paliperidone, Risperidone and Rotenone (Abcam), DehydroAripiprazole (Santa Cruz Biotechnology), DidesmethylCariprazine (Clearsynth), Haloperidol, Iloperidone, Lurasidone, Zotepine (Sigma), Brexpiprazole and Cariprazine (Cayman Chemicals) were dissolved to desired stock concentrations in DMSO. Chlorpromazine (Sigma) was dissolved in distilled water, while Antimycin A, FCCP, Oligomycin A (Sigma) and Piericidin A (Cayman Chemicals) were dissolved in ethanol. All drug stock solutions were diluted into appropriate cell culture media to desired concentrations.
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2

GPCR Ligand-Binding Assay Protocol

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Serotonin (5-HT), olanzapine and aripiprazole were purchased from Sigma-Aldrich, risperidone, clozapine and cariprazine were purchased from Cayman Chemical Company and haloperidol was purchased from Tocris bioscience. The BRET 2 substrates, coelenterazine 400a or DeepBlueC and [methoxy e-Coelenterazine (Me-O-e-CTZ)] or Prolume Purple were from Nanolight TM Technology.
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