Tumour and normal tissues were embedded in paraffin and cut into 4 µm-thick sections. The sections were de-paraffinised using xylene and rehydrated using a gradient concentration of ethanol; the antigen was extracted using microwaves. Subsequently, the sections were incubated with anti-KRT5 (ab8068; Abcam) at 4°C overnight and then with a secondary antibody (ab288151; Abcam) at room temperature for 30 min. DAB plus substrate (D7679, Sigma-Aldrich) was added and the sections were incubated for 10 min. The results were visualised using a microplate reader.
D7679
Manufactured by Merck Group
D7679 is a laboratory equipment item produced by Merck Group. It is designed for general laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to give a concise and accurate description of this product.
Automatically generated - may contain errors
2 protocols using d7679
1
Immunohistochemical analysis of KRT5 in tissues
2
Microscopic Visualization of Cellular Structures
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A work solution of NBT/BCIP (Sigma-Aldrich, B3679) and DAB (Sigma-Aldrich, D7679) was diluted accordingly to the manufacturer's recommendations. The sections were observed, and photos were taken using a CX-41 phase-contrast microscope (×1000, Olympus America Inc., Melville, NY, USA).
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