The mice were sacrificed under anaesthesia after FMT. The whole intestine was isolated and rinsed with ice-cold sterile PBS solution. The small intestine was divided into proximal, middle and distal segments on average, with the colorectum as the fourth segment. Intestinal tumour burden was recorded according to its intestinal site and maximum diameter by an Olympus SZX7 stereo dissecting microscope. The tumour burden was based on gross counts. The proximal tissue of each intestinal segment was snap frozen in liquid nitrogen and kept at −80 °C later and the distal tissue was fixed with 4% paraformaldehyde.
Paraffin-embedded intestinal tissue was cut into 5 μm slices for H&E staining. Pathological types of specimens were diagnosed by two experienced pathologists who were unaware of the treatment allocation of the mice.
Paraffin-embedded intestinal tissue was cut into 5 μm slices for H&E staining. Pathological types of specimens were diagnosed by two experienced pathologists who were unaware of the treatment allocation of the mice.