Piperazine erastin

Erastin (#HY-15763), (1S,3R)-RSL3 (RSL3, #HY-100218A), Ferrostatin-1 (#HY-100579), Z-VAD-FMK (#HY-16658B), Necrosulfonamide (#HY-100573), 6-Diazo-5-oxo-L-nor-Leucine (DON, #HY-108357), UDP-GlcNAc Disodium Salt (UDP-GlcNAc, #HY-112174), Cycloheximide (CHX, #HY-12320), MG-132 (#HY-13259) and Piperazine Erastin (#HY-100887) were purchased from Med Chem Express (MCE, USA). Benzyl 2-acetamido-2-deoxy-a-D-galactopyranoside (BADGP, B4894) was purchased from Sigma-Aldrich (USA). Thiamet-G (TMG, S7213) was purchased from Selleck Chemicals (USA). Human OGT, GFPT1 and ZEB1 knockdown plasmids were cloned into the pPLK GFP lentivirus vector (PPL, China) and FV055 lentivirus vector (Fubio, China), respectively. FV026 lentivirus vector (Fubio, China) was used to overexpress ZEB1-WT and ZEB1 mutant (T678A or S555A). The shRNA and overexpression target sequences are listed in Table S1 and 2.

(1S,3R)-RSL3 (RSL3, #HY-100218A), erastin (#HY-15763), ferrostatin-1 (#HY-100579), Z-VAD-FMK (#HY-16658B), necrostastin-1 (#HY-15760), necrostatin-2 (#HY-14622), GSK2606414 (#HY-18072), and piperazine erastin (#HY-100887) were purchased from MedChemExpress (MCE; Monmouth Junction, NJ, USA). Ceapin-A7 (T9110) and 4μ8C (T6363) were purchased from MedChemExpress.

Five-week-old nude mice were purchased from Bikai Laboratory Animal Corp. (Bikai, China). Bel-7402 cells treated as indicated were subcutaneously injected into mice at a density of 5 × 10⁶. After xenografts were formed, piperazine erastin, which was purchased from MedChemExpress, was subcutaneously injected into the mice once a day. The tumors were measured 14 days after piperazine erastin injection, and tumor volumes were calculated with the formula 0.5 × L × W², where L represents the length and W represents the width. The animal experiments were conducted under the institutional guidelines of Shanghai Children’s Medical Center.